摘要
【目的】揭示鹅掌楸属遗传结构和地理变异特点,为鹅掌楸属遗传资源的保存、利用及改良提供依据。【方法】以9个鹅掌楸种源97份样本和4个北美鹅掌楸种源46份样本为材料,采用RAD-seq测序鉴定各样本SNP标记,计算观测杂合度(Ho)、期望杂合度(He)、核苷酸多样性(π)和基因分化系数(Gst)等遗传统计量指标,分析鹅掌楸属种源遗传多样性和遗传结构。【结果】在143份鹅掌楸属样本中共鉴定出4454个高质量的SNP标记;鹅掌楸种源之间存在较大的遗传分化以及中水平的基因流(Gst=0.2419、Nm=0.8051),北美鹅掌楸种源之间存在很大的的遗传分化以及低水平的基因流(Gst=0.3886>0.25、Nm=0.3970);通过结构分析将13个鹅掌楸属种源分为3个类群,其中9个中国的鹅掌楸种源被分为东部种源群(即类群2)和西部种源群(即类群1),类群3均为北美鹅掌楸,遗传多样性顺序为:类群3>类群1>类群2。【结论】鹅掌楸属遗传结构的形成与其地理隔离和片段化分布有关,且存在由于小群体效应和片段化影响导致的濒危现象,本研究开发的SNP标记可为鹅掌楸属分子鉴定、种质创新及种质资源收集与保存提供参考。
【Objective】This study aims to reveal the genetic structure and geographic variation characteristics of Liriodendron populations,in order to provide a basis for the conservation,utilization and improvement of genetic resources of Liriodendron.【Method】A total of 97 samples derived from 9 provenances of Liriodendron chinensis and 46 samples derived from 4 provenances of L.tulipifera were collected.The SNP markers of the samples were identified by RAD-Seq sequencing.The observed heterozygosity(Ho),expected heterozygosity(He),nucleotide diversity(π),gene differential coefficient(Gst)and other genetic statistics were calculated to analyze the genetic diversity and genetic structure of Liriodendron provenances.【Result】A total of 4454 high quality SNP markers were identified in the 143 samples of Liriodendron.There were Liriodendron maintained moderate genetic differentiation and medium level gene flow among provenances of L.chinensis(Gst=0.2419,Nm=0.8051).There were large genetic differentiation and low level gene flow among L.tulipifera provenances(Gst=0.3886>0.25,Nm=0.3970).The 13 Liriodendron provenances were divided into 3 subgroups by population structure analysis.Among them,9 provenances of L.chinensis were divided into eastern provenances(group 2)and western provenances(group 1),and the other provenances belonged to group 3 and they are all L.tulipifera.The order of genetic diversity was:group 3>group 1>group 2.【Conclusion】The formation of genetic structure of Liriodendron is related to its geographical isolation and fragmented distribution,and there are endangered phenomena caused by small population effect and fragmentation.The SNP markers developed in this study can provide a reference basis for molecular identification,germplasm innovation and collection and preservation of germplasm resources of Liriodendron.
作者
潘文婷
孙建军
原勤勤
张利利
邓康桥
厉月桥
Pan Wenting;Sun Jianjun;Yuan Qinqin;Zhang Lili;Deng Kangqiao;Li Yueqiao(Subtropical Experimental Center, CAF Fenyi 336600)
出处
《林业科学》
EI
CAS
CSCD
北大核心
2022年第4期74-81,共8页
Scientia Silvae Sinicae
基金
中央级公益性科研院所基本科研业务费专项资金(CAFYBB2016QA015)。
