摘要
目的研究氢化二氧化钛纳米管对小鼠成骨前体细胞(MC3T3-E1)和单核巨噬细胞(RAW264.7)增殖黏附的影响。方法利用阳极氧化法在空白组纯钛(Ti)表面制备纳米管。空气中烧结形成锐钛矿晶型(air-TNTs)为对照组,将air-TNTs高温氢化形成氢化二氧化钛纳米管(H_(2)-TNTs)为实验组,观察材料表征,并在三组材料表面分别孵育RAW264.7和MC3T3-E1,观察细胞增殖和形态。结果H_(2)-TNTs表面RAW264.7细胞在孵育后第24 h和48 h吸光度值增加,但显著低于Ti(P<0.05)。H_(2)-TNTs表面MC3T3-E1细胞在孵育的第3 d和第5 d,吸光度值显著高于air-TNTs和Ti(P<0.05)。随孵育时间延长,RAW264.7在Ti表面逐渐变成多角形,而在air-TNTs和H_(2)-TNTs表面仍以圆形细胞为主。MC3T3-E1在H_(2)-TNTs表面明显伸长。结论氢化二氧化钛纳米管相对抑制RAW264.7的增殖,促进MC3T3-E1的增殖和早期铺展。
Objective To investigate the effect of hydrogenated TiO_(2) nanotube films on the proliferation and adhesion of mouse embryo osteoblast precursor cells(MC3T3-E1)and the mouse macrophages(RAW264.7).Methods Titanium dioxide nanotubes(TNTs)were hydrogenated anodized by a thermal treatment.There were three groups,hydrogenated TNTs(H_(2)-TNTs),unmodified TNTs(air-TNTs),and Ti substrates(Ti).The morphology,roughness,wettability,and polarization curve were measured.The cell proliferation on different substrates was examined and the morphology of the cells was observed with fluorescence microscope.Results The number of Raw264.7 cells on the H_(2)-TNTs samples was significantly lower than that of the Ti group at 24 h and 48 h(P<0.05).MC3T3-E1 cell proliferation on the H_(2)-TNTs surface was greater than that on the air-TNTs and Ti substrates from the third day of incubation(P<0.05).The RAW264.7 cells appeared to have a round shape on air-TNTs and H_(2)-TNTs.The MC3T3-E1 cells on the H_(2)-TNTs sample had a more elongated shape than those on the air-TNTs.Conclusions The H_(2)-TNTs could promote the proliferation of MC3T3-E1 cells but inhibit that of Raw264.7 cells compared with the air-TNTs and Ti substrates.
作者
卢燃
王彩云
曹旭
陈溯
LU Ran;WANG Cai-yun;CAO Xu;CHEN Su(VIP Department,Capital Medical University School of Stomatology,Beijing 100050,China)
出处
《北京口腔医学》
CAS
2022年第3期176-181,共6页
Beijing Journal of Stomatology
基金
国家自然科学基金(81570999)。
关键词
钛
氢化
纳米管
亲水性
巨噬细胞
Titanium
Hydrogenation
Nanotubes
Hydrophilicity
Macrophage
