摘要
通过分析宿根矮化病抗感甘蔗品种蔗茎内生真菌和细菌的群落特征,旨在了解内生真菌和细菌类群在寄主抗病中的作用,从而为构建生态防控宿根矮化病提供理论依据。利用Illumina MiSeq高通量测序技术,比较了宿根矮化病感病品种YT60和抗病品种HoCP95988蔗茎内生真菌及细菌的多样性指数、类群组成和物种差异。感病品种YT60蔗茎内生真菌和细菌的Alpha多样性指数高于抗病品种HoCP95988,具有更多的物种组成。蔗茎内生细菌主要分布在变形菌门(Proteobacteria),子囊菌门(Ascomycota)和担子菌门(Basidiomycota)为内生真菌的优势菌门。抗感病甘蔗品种蔗茎内生真菌和细菌种群组成差异明显,其中内生细菌在浮霉菌门(Planctomycetes)、绿弯菌门(Chloroflexi)、芽单胞菌门(Gemmatimonadetes)和软壁菌门(Tenericutes)中包含的34个属的物种丰度存在显著差异,并且差异物种在感病品种YT60中呈现较高相对丰度。宿根矮化病抗感甘蔗品种茎部内生真菌和细菌多样性均较为丰富,种群组成存在较大差异,差异物种的分类信息为宿根矮化病生防微生物资源的挖掘提供了线索。
By analyzing the structural characteristics of endophytic fungi and bacteria in the stalks of sugarcane varieties resistant to ratoon stunting disease(RSD),the aim is to understand the roles of endophytic fungi and bacteria in the resistance of sugarcane,so as to provide theoretical basis for ecological control of RSD.Using Illumina MiSeq high-throughput sequencing method,the communities,diversity index,and differences of endophytic bacteria and fungi between susceptible variety YT60 and resistant variety HoCP95988 were compared.The Alpha diversity indexes of endophytic fungi and bacteria in the stalks of susceptible cultivar YT60 was higher than that of resistant cultivar HoCP95988,indicating more species composition.The endophytic bacteria mainly distributed in Proteobacteria,while Ascomycota and Basidiomycota were the dominant phyla of endophytic fungi.The species compositions of 34 genera in 4 phyla Planctomycetes,Chloroflexi,Gemmatimonadetes,and Tenericutes between susceptible and resistant varieties were significantly different.Among them,the relative abundance of different species was higher in susceptible variety YT60.The diversity of endophytic fungal and bacterial communities in the stalks of sugarcane varieties resistant to RSD were relatively rich,and there were significant differences in the composition.Meanwhile,the classification information of different species provided clues to explore the biocontrol microbial resources for control RSD.
作者
高小宁
刘睿
吴自林
吴嘉云
GAO Xiao-ning;LIU Rui;WU Zi-lin;WU Jia-yun(Institute of Nanfan&Seed Industry,Guangdong Academy of Sciences,Guangzhou 510316;Zhanjiang Sugarcane Research Center,Guangzhou Sugarcane Industry Research Institute,Zhanjiang 524300)
出处
《生物技术通报》
CAS
CSCD
北大核心
2022年第6期166-173,共8页
Biotechnology Bulletin
基金
广东省科学院建设国内一流研究机构行动专项资金项目(2019GDASYL-0103029)
广东省甘蔗剑麻产业技术体系创新团队(2019KJ104-05)
湛江市创新创业团队引育“领航计划”(2020LHJH006)。
关键词
甘蔗
宿根矮化病
内生真菌
内生细菌
多样性指数
种群组成
sugarcane
ratoon stunting disease
endophytic fungi
endophytic bacteria
diversity index
population composition
