摘要
采用亲水色谱-电雾式检测器-电喷雾-飞行时间质谱,测定西洋参不同部位(芦头、主根、参须)中多糖的单糖组成。采用超声辅助酸解法对西洋参多糖进行完全水解,应用Waters Xbridge Amide柱(4.6 mm×250 mm,5μm),梯度洗脱(A为乙酸铵水溶液,B为乙腈),流速0.8 mL/min,柱温40℃,ESI-TOF/MS进行定性鉴别,CAD检测器(雾化器温度60℃,N_(2)压力8.8 kPa)进行定量测定,结合主成分分析对西洋参不同部位进行区分。该方法在一定质量浓度范围内具有良好的线性(相关系数>0.9991),检出限为0.03~0.13μg/mL,平均回收率为94.17%~107.15%。应用该方法测定了西洋参不同部位多糖水解产物中7种单糖(葡萄糖、果糖、阿拉伯糖、鼠李糖、木糖、岩藻糖、甘露糖)和2种糖醛酸(半乳糖醛酸、葡萄糖醛酸),主根中葡萄糖含量较高,其平均值为(2.123±0.070)mg/g;芦头中鼠李糖、阿拉伯糖、果糖、甘露糖、葡萄糖醛酸、半乳糖醛酸含量较高,其平均值分别为(0.037±0.006)mg/g、(0.179±0.023)mg/g、(0.158±0.028)mg/g、(0.036±0.006)mg/g、(0.069±0.007)mg/g、(0.376±0.096)mg/g;参须中岩藻糖含量较高,其平均值为(0.120±0.005)mg/g。该方法简单,灵敏度、重复性好,为西洋参单糖组成和不同药用部位开发利用提供了方法和数据支持。
The study aimed to determine the monosaccharide composition of polysaccharides from different parts(such as rhizome,taproot,and fibrous ginseng root)of Panacis Quinquefolii Radix using HILIC-CAD-ESI-TOF/MS.The polysaccharides of Panacis Quinquefolii Radix were completely hydrolyzed via ultrasound-assisted acid hydrolysis,and a Waters Xbridge Amide column(4.6 mm×250 mm,5μm)with gradient elution(A for aqueous ammonium acetate solution,B for acetonitrile)at a flow rate of 0.8 mL/min,column temperature of 40℃was used.ESI-TOF/MS was used for qualitative identification,and CAD detector(with a nebulizer temperature of 60℃and N_(2) pressure of 8.8 kPa)was used for quantitative determination,combined with principal component analysis to differentiate the different parts of Panacis Quinquefolii Radix.The suggested method showed a good linearity in the corresponding mass concentration ranges(correlation coefficients>0.9991);the detection limit was found to be between 0.03μg/mL and 0.13μg/mL,and recoveries between 94.17%and 107.15%were achieved.The method was applied to determine the presence of seven monosaccharides(glucose,fructose,arabinose,rhamnose,xylose,fucose,and mannose)and two uronic acids(galacuronic and glucuronic acids)in the products obtained by subjecting the different parts of Panacis Quinquefolii Radix to polysaccharide hydrolysis.In the taproot,the content of glucose was relatively high with a mean of(2.123±0.070)mg/g;in the rhizome,the contents of rhamnose,arabinose,fructose,mannose,glucuronic acid,and galacturonic acid were relatively high with a mean of(0.037±0.006)mg/g,(0.179±0.023)mg/g,(0.158±0.028)mg/g,(0.036±0.006)mg/g,(0.069±0.007)mg/g,and(0.376±0.096)mg/g,respectively;and in the fibrous ginseng root,the fucose content was relatively high with a mean of(0.120±0.005)mg/g.The suggested method was found to be simple,sensitive,and reproducible and provided method and data support for the composition of monosaccharides of Panacis Quinquefolii Radix and the exploitation of different medicinal parts.
作者
赵志国
张敏敏
李月
王岱杰
杨国红
王晓
赵恒强
ZHAO Zhi-guo;ZHANG Min-min;LI Yue;WANG Dai-jie;YANG Guo-hong;WANG Xiao;ZHAO Heng-qiang(Shandong Analysis and Test Center;School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China)
出处
《山东科学》
CAS
2022年第4期1-8,共8页
Shandong Science
基金
中央本级重大增减支项目(2060302)
山东省重点研发计划(2019GSF108062)
齐鲁工业大学(山东省科学院)科教产融合创新试点工程项目(国际合作)(2020KJC-GH08)
山东省外专双百计划
山东省泰山学者岗位资助(郭兰萍)
威海市产学研战略合作“特聘专家”资助计划。
