摘要
研究亚高山绣球菌多糖的提取纯化、结构表征及抗炎作用。采用响应曲面法优化提取工艺,柱层析等方法纯化亚高山绣球菌多糖(Sparassis subalpina polysaccharide,SSP);采用气相色谱、核磁共振谱和电子显微镜等表征结构,实时荧光定量PCR等分析抗炎活性。结果显示,在最佳提取条件(液料比80 mL/g,提取时间60 min,提取温度70℃)下,总糖提取率为(18.21±0.68)%。SSP重均分子质量是50 kDa,由葡萄糖、甘露糖、半乳糖和阿拉伯糖组成(6.5∶1.3∶1∶1),重复结构单位是→3)-α-D-Galp-(1→2)-β-D-Glup-(1→3)-β-L-Araf-(1→3)-α-D-Manp-(1→,呈无分支的网状结构。SSP对炎症细胞中TNF-α、COX-2和iNOS的表达均有显著抑制作用(P<0.05)。结果表明无分支网状结构SSP具有抗炎作用。
To study the isolation,purification,structure properties and anti-inflammatory activities of polysaccharides from Sparassis subalpina.Response surface methodology was used to obtain the optimal processing conditions of total sugar from S.subalpina and the method of column chromatography was used for the purification of a S.subalpina polysaccharide(SSP).Using gas chromatography,NMR spectroscopy and electron microscopies,the structure was studied.Anti-inflammatory activity was determined using RT-qPCR,et al.The results showed that the experimental yield was(18.21±0.68)%under the optimal processing conditions(liquid to solid ratio 80 mL/g,extraction time 60 min,extraction temperature 70℃).The average molecular weight of SSP was calculated to be 50 kDa and SSP consisted of glucose,mannose,galactose and arabinose with a molar ratio of 6.5∶1.3∶1∶1.The repeated unit in SSP was determined as→3)-α-D-Galp-(1→2)-β-D-Glup-(1→3)-β-L-Araf-(1→3)-α-D-Manp-(1→,and SSP showed a non-branched and netted state.SSP demonstrated significant inhibitory effects on the gene expressions of TNF-α,COX-2 and iNOS in LPS-stimulated RAW 264.7 cells(P<0.05).The results evidenced the potential effects of SSP with the non-branched and netted state on anti-inflammatory activities.
作者
罗巅辉
张泽彤
LUO Dian-hui;ZHANG Ze-tong(Department of Bioengineering and Biotechnology,Huaqiao University,Xiamen 361021,China)
出处
《天然产物研究与开发》
CAS
CSCD
2022年第7期1110-1118,共9页
Natural Product Research and Development
基金
国家自然科学基金(31201314)。
关键词
亚高山绣球菌
微观结构
抗炎作用
Sparassis subalpina
microstructure
anti-inflammatory activity
