摘要
N-末端脑利钠肽前体(NT-proBNP)的灵敏检测是降低心力衰竭死亡率的重要先决条件。在这项研究中,开发了一种无标记电流免疫传感器来灵敏地检测NT-proBNP。它基于三金属Au@PdPt纳米颗粒修饰花状MnO_(2)纳米片的复合纳米材料。三金属Au@PdPt纳米颗粒提高了催化活性,花状MnO_(2)纳米片具有大的活性表面积能有效阻止Au@PdPt纳米颗粒聚集。因此,该纳米复合材料表现出较高的催化活性,增强的电子转移能力并有效地固定了抗体,从而放大了电流信号。基于上述优点,制备的免疫传感器表现出从1 pg·mL^(-1)到100 ng·mL^(-1)的宽浓度范围和0.76 pg·mL^(-1)的低检测限。此外,这种无标记免疫传感器具有高灵敏度、良好的选择性和长期稳定性,在生物测定分析中具有广阔的应用前景。
Sensitive detection of N-terminal brain natriuretic peptide(NT-proBNP)is an important prerequisite for reducing the mortality of heart failure.In this study,a label-free current immunosensor was developed to sensitively detect NT-proBNP.It is based on the composite nano material of trimetal Au@PdPt nanoparticles modified with flower-shaped MnO_(2) nanosheets.The three-metal Au@PdPt nanoparticles improve the catalytic activity,and the flower-like MnO_(2) nanosheets have a large active surface area,which can effectively prevent the aggregation of Au@PdPt nanoparticles.Therefore,the nanocomposite material exhibits high catalytic activity,enhances electron transfer and effectively immobilizes the antibody,thereby amplifying the current signal.Based on the above advantages,the prepared immunosensor showed a wide concentration range from 1 pg·mL^(-1) to 100 ng·mL^(-1) and a low detection limit of 0.76 pg·mL^(-1).In addition,this label-free immunosensor has high sensitivity,special selectivity and long-term stability,and has broad application prospects in bioassay analysis.
作者
张斌
王小红
ZHANG Bin;WANG Xiao-hong(School of Chemical Engineering and Technology,Hainan University,Haikou 570228 China;School of Science,Hainan University,Haikou 570228 China)
出处
《化学研究与应用》
CAS
CSCD
北大核心
2022年第7期1596-1602,共7页
Chemical Research and Application
基金
海南省教育厅研究基金项目(2019RC114)资助。
