摘要
[Objectives]To determine and analyze amino acid content in Maca from different habitats and varieties.[Methods]Maca from two habitats and three colors were selected and a kind of turnip commonly used was to fake Maca.After optimizing the hydrolysis conditions,100 mg Maca samples were hydrolyzed in the concentration of 6 mol/L hydrochloric acid at 110℃for 24 h.The content range of amino acids in each sample was detected by HPLC.[Results]The average total content of amino acids in Maca was 9.52 g/100 g.Yunnan yellow Maca contained 16 kinds of amino acids,including 7 kinds of human essential amino acids,accounting for 17.2%of the total amino acids while Yunnan purple Maca held 16 kinds of amino acids,counting 7 kinds of human essential amino acids,accounting for 16.5%of the total amino acids.The amino acid ratio coefficient method also showed that the two kinds of Maca had higher nutritional value than the others.15 kinds of amino acids were detected in Yunnan black Maca,including 6 kinds of essential amino acids,accounting for 9.60%of the total amino acids.Among amino acids in Yunnan Maca,the highest content was Arg.A total of 12 kinds of amino acids,together with 6 essential human amino acids,were determined in Peru black Maca,accounting for 11.6%of the total amino acids.In Peru black Maca the highest concentration of amino acid was Pro.It was noteworthy that the amino acid species were comprehensive in Maca,and high level of amino acids resulted in high nutritional value.Its specific amino acid composition ratio was obviously different from Turnip,the counterfeit goods to deceive customers.[Conclusions]The method of automatic amino acid analyzer is simple and accurate,and can be used for the identification and content determination of amino acids in Maca as amino acid is an essential nutrient in Maca.
基金
Supported by Scientific Research Project of Hunan Provincial Department of Education (21C1516)
the Scientific Research Project of Education Department of Yongzhou City,Hunan Province,China (2020-YZKJ-036)
