期刊文献+

甘蔗原汁后混浊及其蛋白质组成特征 被引量:2

Post-haze and Protein Composition of Raw Sugarcane Juice
下载PDF
导出
摘要 甘蔗原汁后混浊是指澄清甘蔗汁在贮存或商品流通期间发生成分变化,浊度增加或产生沉淀的现象。该研究通过聚丙烯酰胺凝胶电泳、组合式质谱鉴定、氨基酸组分分析和傅立叶红外分析等方法,研究甘蔗原汁后混浊物的基本成分、蛋白质的分子质量大小、种类、氨基酸组成及二级结构。实验发现,甘蔗原汁后混浊物中主要成分是总糖,其含量达60.94%,其次是灰分和蛋白质,含量分别为19.52%和18.43%,酚类仅为0.62%。后混浊蛋白质分子质量分布在42 ku左右和小于20 ku,分别鉴定出12种和44种蛋白,其中丰度较高的几种蛋白分别为protein Z,PREDICTED:calmodulin-7 isoform X1,PREDICTED:calcium-dependent protein kinase 34 isoformX1和hypothetical protein SORBIDRAFT_01g014740。后混浊蛋白质和上清液蛋白质的氨基酸组成差异不大,但在二级结构上差异明显。后混浊蛋白质中除了无规卷曲结构远高于上清蛋白质外,α-螺旋和β-转角分别比上清蛋白质低33.43%和22.42%,而β-折叠差异不大。总的来说,分子质量大小低于42 ku、疏水值高、α-螺旋含量低和无规卷曲含量高的蛋白质更容易参与甘蔗原汁后混浊。 Post-haze of raw sugarcane juice refers to the phenomenon that the clarified raw sugarcane juice undergoes changes in composition,increase of turbidity or precipitation during storage or commodity circulation.In this study,the basic composition as well as the molecular weight,type,amino acid composition and secondary structures of proteins of the sediment in sugarcane juice were studied by polyacrylamide gel electrophoresis,combined mass spectrometry,amino acid analysis and Fourier transform infrared spectroscopy.The experimental results showed that the main component in the sediment of the raw sugarcane juice was sugar(total sugar content 60.94%),followed by ash and protein(19.52%and 18.43%,respectively),and the content of total phenolics was only 0.62%.The molecular weight distribution of post-haze proteins was about 42 ku and lower than 20 ku,and 12 and 44 proteins were identified,respectively.Among them,the proteins with higher abundance are protein Z,PREDICTED:calmodulin-7 isoform X1,PREDICTED:calcium-dependent protein kinase 34 isoformX1,and hypothetical protein SORBIDRAFT_01g014740.The amino acid compositions of the post-haze proteins and the supernatant proteins were only marginally different,but their secondary structures differed significantly.Except that the content of random coil structure in the post-haze proteins was much higher than that of the supernatant protein,theα-helix andβ-turn of post-haze proteins were lower than those of the supernatant protein by 33.43%and 22.42%,respectively,although the difference in theβ-sheet was insignificant.In general,proteins with molecular weight slower than 42 ku,high hydrophobicity,lowα-helix content and high random coil content more likely contributed to the post-haze of raw sugarcane juice.
作者 王琦 蒙丽丹 田润 王淑培 谢彩锋 杭方学 李凯 WANG Qi;MENG Lidan;TIAN Run;WANG Shupei;XIE Caifeng;HANG Fangxue;LI Kai(College of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China;College of Environmental and Life Sciences,Nanning Normal University,Nanning 530001,China;Sugar Industry and Comprehensive Utilization Research Center of Ministry of Education,Guangxi Sugar Association,Nanning 530004,China)
出处 《现代食品科技》 CAS 北大核心 2022年第7期143-151,共9页 Modern Food Science and Technology
基金 国家现代农业产业技术体系资助(CARS-170502) 蔗糖产业省部共建协同创新中心 广西绿色制糖工程技术研究中心(2017GCZX)。
关键词 甘蔗原汁 后混浊 氨基酸 疏水性 二级结构 sugarcane juice post-haze amino acids hydrophobicity secondary structure
  • 相关文献

参考文献6

二级参考文献113

  • 1吴琪,朱珉.啤酒浊度测定方法及影响因素的探讨[J].食品与发酵工业,1994,20(4):36-43. 被引量:3
  • 2陈有容,刘庆玮.酱油中沉淀及混浊物产生的原因初探[J].天津微生物,1989(2):32-37. 被引量:14
  • 3林峰,白少勇,邹慧君,谢广发,周建弟.黄酒蛋白质沉淀[J].酿酒科技,2005(9):69-72. 被引量:12
  • 4杨国军,俞关松,尉冬青.黄酒中蛋白质分布及含量与酒质稳定性关系的研究[J].中国酿造,2005,24(10):47-49. 被引量:13
  • 5BAMFORTH C W. Beer haze[J]. Arn Soc Brew Chem, 1999, 57: 81- 90.
  • 6SIEBERT K J. Effects of protein-polyphenol interactions on beverage haze, stabilization and analysis[J]. Agric Food Chem, 1999, 47(2): 353- 362.
  • 7BOIVIN P, MARTEL C. Proteolysis during malting[J]. Ferment, 1991, 4: 182-186.
  • 8EVANS D E, HEJGAARD J. The impact of malt derived proteins on beer foam quality. Part 1. The effect of germination and kilning on the level of protein Z4, protein Z7 and LTP1 [J]. Inst Brew, 1999, 105: 159- 169.
  • 9SLACK P T, BAMFORTH C W. The fractionation of polypeptides from barley and beer by hydrophobic interaction chromatography: the influence of their hydrophobieity on foam stability[J]. Inst Brew, 1983, 89: 379-401.
  • 10SIEBERT K J, LYNN P Y. Mechanisms of beer colloidal stabilization [J]. Am Soc Brew Chem, 1997, 55: 73-78.

共引文献50

同被引文献27

引证文献2

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部