摘要
利用响应面法优化刺梨中质量标记物的提取工艺,并建立HPLC法同时测定其含量。以刺梨根为原料,利用单因素实验与响应面试验优化了刺梨中4个质量标志物(三萜类化合物)的提取工艺,并比较分析刺梨不同部位(根、茎、叶子和果实)的质量标志物含量差异。结果表明,单因素实验确定了影响刺梨萜类物质得率的重要因素为溶剂用量、乙醇浓度、提取时间和提取次数。以刺梨中4个质量标志物的总含量为综合考察指标,采用响应面试验确定了刺梨三萜化合物的最适提取工艺条件为:按照溶剂用量为1:25 g/mL加入体积分数为70%的乙醇,先超声30 min,再将料液进行加热回流2 h,在此条件下,刺梨叶中4个质量标志物的总含量为2.64%,其中刺梨苷含量为0.99%、野蔷薇苷含量为0.72%、蔷薇酸含量为0.43%、委陵菜酸含量为0.51%;刺梨不同部位的4个质量标志物总含量为刺梨叶>刺梨根>刺梨茎>刺梨果。本研究为刺梨的质量评价及其总三萜的开发利用提供科学参考。
Extraction process for Q-markers from Rosa roxburghii using response surface methodology was optimized and HPLC method was established for simultaneous determination of their content.The extraction process of Q-marker from the root of Rosa roxburghii Tratt was optimized by single factor experiments and response surface methodology,and the four Q-markers(triterpenoids)in different parts(root,stem,leaf and fruit)of Rosa roxburghii Tratt were analyzed.Results showed that,through single factor experiments,the important factors affecting the yield of Q-markers were determined as solvent dosage,ethanol concentration,extraction time and extraction times.Response surface methodology was used to optimize the extraction process of Q-markers from Rosa roxburghii.The optimal extraction conditions were as follows:The solvent dosage was 1:25 g/mL,70%ethanol was added,the extraction time was 30 min,and then the material was refluxed for 2 h.The yield of four Q-markers from the Rosa roxburghii leaves was 2.64%,including 0.99%of Kaji-ichigoside F1,0.72%mg/g of rosamultin,0.43%of rosolic acid and 0.51%of 2α,19α-dihydroxyursolic acid.The total content of Q-markers in different parts of Rosa roxburghii Tratt was in the order of leaves,roots,stems and fruits.This study would provide scientific reference for quality evaluation of Rosa roxburghii and development and utilization of total triterpenoids.
作者
汪涛
李良群
严艳芳
田强
陈发菊
谭云飞
杨娟
彭梅
杨礼寿
罗忠圣
李齐激
杨小生
WANG Tao;LI Liangqun;YAN Yanfang;TIAN Qiang;CHEN Faju;TAN Yunfei;YANG Juan;PENG Mei;YANG Lishou;LUO Zhongsheng;LI Qiji;YANG Xiaosheng(Graduate School of Guizhou University of Chinese Medicine,Guiyang 550025,China;The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550014,China;The Key Laboratory of Chemistry for Natural Products of Guizhou Medical University,Guiyang 550014,China)
出处
《食品工业科技》
CAS
北大核心
2022年第14期251-260,共10页
Science and Technology of Food Industry
基金
贵州省科技创新能力建设专项:“贵州食药用资源康养产品研发与成果转化创新能力提升平台建设项目”(黔科合服企[2020]4013号)
贵州省工信厅刺梨专项:“刺梨核心功效及健康养生产品研究与开发”(黔财农[2020]307号)
贵州省教育厅高等学校特色重点实验室项目:“贵州省高等学校特色重点实验室”(黔教合KY字[2020]018号)
贵州省科技计划项目(黔科合支撑[2022]一般171,[2020]1Y148,黔科合基础-ZK[2021]一般512)。
关键词
刺梨
质量标志物
三萜化合物
提取工艺
含量同时测定
Rosa roxburghii
quality marker
triterpenes
extraction process
simultaneous content determination
