摘要
以欧亚香花芥和河北雾灵山、塞罕坝的2个野生型雾灵香花芥为试验材料,研究其功能叶片的形态、解剖结构、色素含量、叶绿素荧光及光合参数,借助相关性和冗余分析的方法探究两种香花芥属植物叶片结构与光合生理的关系。结果表明:1)2个野生型雾灵香花芥的叶片结构和光合生理存在种内差异,其中雾灵山植株叶片加厚、叶面积增大,栅栏组织加厚,色素含量丰富,净光合速率高,而塞罕坝植株叶片厚、气孔密度大,海绵组织加厚,导管、筛管直径加粗,PSⅡ反应中心活性参数高,蒸腾速率强;欧亚香花芥的叶表毛状体分布密集,栅海比高,色素含量丰富,净光合速率及水分利用效率高,均优于2个野生型雾灵香花芥。2)两种香花芥属植物叶片结构性状与光合生理参数间存在较强相关性,借助冗余分析筛选出栅海比、海绵组织厚度、表皮毛密度、组织结构疏松度、叶面积、导管直径、栅栏组织厚度和维管束直径等对光合生理有重要影响的叶片结构参数,其中栅海比达到显著水平(P<0.05),是两种香花芥属植物生态适应性及资源获取的主要驱动因素。总的来说,两种香花芥属植物均在引种地长势良好,具备一定的园林开发基础;同时栅海比可作为香花芥属植物筛选高光效优良种质的重要参考指标。
This research studied the functional leaf morphology,anatomical structure,pigment content,chlorophyll fluorescence and photosynthetic parameters of Hesperis matronalis and wild-type Hesperis sibirica from two localities(Wuling Mountain and Saihanba)in Hebei Province.The relationships between leaf structure and photosynthetic physiology of the two species of Hesperis were explored using correlation and redundancy analysis methods.It was found that:1)There were intraspecific differences in leaf structure and photosynthetic physiology of the two wildtype H.sibirica collections.The H.sibirica from Wuling Mountain had comparatively greater leaf thickness(LT),larger leaf area(LA),thicker palisade parenchyma thickness(PPT),richer pigment content,and higher net photosynthetic rate(Pn).The H.sibirica from Saihanba had comparatively high leaf stomatal density(SD),greater sponge parenchyma thickness(SPT),increased diameters of vessel and sieve tubes(VD and STD),high activity parameters for the PSⅡreaction center,and strong transpiration rate(Tr).H.matronalis leaves had high epidermal trichome density(ETD),a high ratio of palisade parenchyma thickness to sponge parenchyma thickness(PPT∶SPT),rich pigment content,and high Pnand water use efficiency(WUE),when compared with values for H.sibirica.2)There was a strong correlation between the leaf structure and the photosynthetic physiological parameters of the two species of Hesperis.Important links between leaf structure and photosynthetic physiological differences and were identified by redundancy analysis,involving the parameters PPT∶SPT,SPT,ETD,spongy ratio,LA,VD,PPT and VBD.Among these,the effect of PPT∶SPT(P<0.05)was mathematically largest and provided the strongest single-trait association with differences in the ecological adaptability and resource acquisition of the two Hesperis species.Overall,the two species of Hesperis performed well at the sites where they were introduced and showed promise for development and utilization as garden plants.The PPT∶SPT could be used as a reference indicator trait for screening high-light-efficiency germplasm lines of Hesperis.
作者
董梦宇
王金鑫
吴萌
周子瑶
程顺
李彦慧
DONG Meng-yu;WANG Jin-xin;WU Meng;ZHOU Zi-yao;CHENG Shun;LI Yan-hui(College of Landscape and Tourism,Hebei Agricultural University,Baoding 071000,China;Saihanba Mechanized Forest Farm of Hebei Province,Chengde 067000,China)
出处
《草业学报》
CSCD
北大核心
2022年第7期172-184,共13页
Acta Prataculturae Sinica
基金
“十三五”国家重点研发计划(2020YFD1000700)
河北省科技厅重点研发计划项目(21326802D)
河北省林业与草原局科技项目(1809508)资助。
关键词
雾灵香花芥
欧亚香花芥
叶片
解剖结构
光合特性
Hesperis sibirica
Hesperis matronalis
leaf
anatomical structure
photosynthetic characteristics
