摘要
目的通过生物信息学分析探索肝细胞癌(hepatocellular carcinoma,HCC)的异常表达基因及其与HCC预后的关系。方法从基因表达综合数据库中选择HCC相关的5个数据集,探索差异表达基因(differentially expressed genes,DEGs),再进一步进行基因本体分析和京都基因与基因组百科全书分析。选择共上调的基因CNIH4及TOMM40L,探索其在HCC组织和正常肝组织中的表达差异(癌症基因组图谱数据库),并探索其表达与患者5年生存的关系。同时收集广东省第二人民医院的8例HCC组织及其癌旁组织,验证CNIH4及TOMM40L mRNA的表达差异。结果本研究发现共上调基因25个,共下调基因21个。基因本体分析和京都基因与基因组百科全书分析结果示DEGs主要与分解代谢、细胞分裂、DNA复制、修复等有关。癌症基因组图谱数据库分析结果表明,CNIH4 mRNA和TOMM40L mRNA在HCC组织中的表达相较于正常肝组织上调(P<0.05),并且二者高表达组患者的5年生存情况较低表达组更差(P<0.05)。临床样本结果表明,CNIH4 mRNA和TOMM40L mRNA在HCC组织中的表达较癌旁组织上调。结论CNIH4、TOMM40L mRNA在HCC组织中表达上调,且其高表达与不良预后相关,可能是HCC潜在的生物标志物及预后指标。
Objective To explore the aberrantly expressed genes in hepatocellular carcinoma(HCC)and their relationship with prognosis of HCC through bioinformatics analysis.Methods Five datasets related to HCC were selected from the Gene Expression Omnibus database to explore differentially expressed genes(DEGs),followed by further gene ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis.The coupregulated genes CNIH4 and TOMM40 were selected to explore the differences in their expressions in HCC tissues and normal tissues,and to explore the relationship between their expressions and the 5-year survival of patients by using TCGA database.Tissues and paraneoplastic tissues of eight cases of HCC who underwent surgery at the Guangdong Second Provincial General Hospital were collected to verify the expression differences of CNIH4 and TOMM40L mRNA.Results A total of 25 up-regulated genes and 21 down-regulated genes were identified in this study.The results of GO analysis and KEGG analysis indicated that DEGs were mainly related to catabolism,cell division,DNA replication and repair.The results of TCGA database analysis showed that the expression of up-regulated genes CNIH4 mRNA and TOMM40L mRNA were up-regulated in HCC tissues as compared with normal tissues(P<0.05)and that the 5-year survival of patients in the high expression group was worse than that in the low expression group(P<0.05).The results of clinical samples showed that CNIH4 mRNA and TOMM40L mRNA were up-regulated in HCC tissues as compared with paraneoplastic tissues.Conclusion CNIH4 and TOMM40L genes are up-regulated in HCC tissues,and their high expressions are associated with poor prognosis,and may be potential biomarkers and prognostic indicators for HCC.
作者
姜敏
赵玉军
JIANG Min;ZHAO Yujun(The Second Department of General Surgery,Guangdong Second Provincial General Hospital,Guangzhou 510317,P.R.China)
出处
《中国普外基础与临床杂志》
CAS
2022年第7期904-909,共6页
Chinese Journal of Bases and Clinics In General Surgery
