摘要
本实验对比了商品化酶联免疫吸附法、荧光免疫层析快速定量法和胶体金快速免疫层析快速定量法中不同提取溶剂对花生粕中黄曲霉毒素B_(1)检测结果的影响,旨在商品化黄曲霉毒素B_(1)的快速检测产品中增加花生粕样本的适用性,以满足食品加工企业、饲料加工企业以及花生粕原料贸易商对花生粕中黄曲霉毒素B_(1)快速检测的需求。结果显示,商品化黄曲霉毒素B_(1) ELISA检测检测试剂盒、黄曲霉毒素B_(1)荧光快速定量检测试纸条和黄曲霉毒素B_(1)胶体金快速定量检测试纸条在花生粕检测中的最优提取溶剂均为30%乙腈-水溶液,食品加工企业、饲料加工企业以及花生粕原料贸易可使用此方法对花生粕中黄曲霉毒素B_(1)进行快速检测。
In this study,the effects of different extraction buffer on the detection results of aflatoxin B_(1)in peanut meal by commercial enzyme-linked immunosorbent assay,fluorescence immunochromatographic rapid quantitative assay and colloidal gold rapid quantitative assay were compared.It aims to increase the applicability of the matrix of peanut meal to the commercial rapid detection products of aflatoxin B_(1)and meet the requirements of food processing enterprises,feed processing enterprises and peanut meal raw material traders for the rapid detection of aflatoxin B_(1)in peanut meal.The results showed that the optimal extraction buffer of commercial aflatoxin B_(1)ELISA detection kit,aflatoxin B_(1)fluorescence rapid quantitative detection strip and aflatoxin B_(1)colloidal gold rapid quantitative detection strip in peanut meal detection was 30%acetonitrile-aqueous solution.Food processing enterprises,feed processing enterprises and raw material trade of peanut meal can use this method for rapid detection of aflatoxin B_(1)in peanut meal.
作者
张君超
田秀梅
王艳
闵国春
靳曼
陈年
ZHANG Junchao;TIAN Xiumei;WANG Yan;MIN Guochun;JIN Man;CHEN Nian(Beijing Meizheng Bio-Tech Co.,Ltd.,Beijing 102200,China;Xiaogan Public Inspection and Testing Center of Hubei Province,Xiaogan 432000,China;Three Gorges Public Inspection and Testing Center,Yichang 443000,China;Liaoning Grain Inspection and Testing Center,Shenyang 110032,China)
出处
《食品安全导刊》
2022年第20期62-64,70,共4页
China Food Safety Magazine
