摘要
为开发长吻(Leiocassis longirostris)生长性状相关的分子标记,为其分子辅助育种提供基础资料,以115尾长吻为研究对象,运用57个单核苷酸多态性标记(SNPs)位点与体重、全长、体长和头高进行关联分析。结果显示,有10个SNPs位点与生长性状显著相关,其中3个SNPs位点(Cluster-65_137265、Cluster-65_111833和Cluster-65_137642)对所测量的4个生长性状均有显著影响(P<0.05);3个SNPs位点(Cluster-65_120392、Cluster-65_5592_0和Cluster-65_105077)对体重、全长和体长有显著影响(P<0.05);Cluster-65_110382对全长、体长和头高有显著影响(P<0.05);Cluster-65_19497和Cluster-24304_1对全长和体长有显著影响(P<0.05);Cluster-65_130153对体长和头高有显著影响(P<0.05)。此外,Cluster-65_137265、Cluster-65_111833、Cluster-65_110382和Cluster-65_137642分别与阴离子交换蛋白2样亚型X1 (anion exchange protein 2-like isoform X1)神经突起导向因子4样(netrin-4-like)、酪氨酸蛋白激酶Tec亚型X2 (tyrosine-protein kinase Tec isoform X2)和氨甲酰磷酸合成酶1 (carbamoyl-phosphate synthase [ammonia], mitochondrial, CPS1)相似度最高,表明这4个基因可能参与调控了长吻的生长。对与生长相关的10个SNPs位点进行多态性检测,平均观测杂合度和平均期望杂合度分别为0.537和0.467,平均多态信息含量为0.357。本研究为长吻的遗传改良和选择育种提供了基础资料,并首次发现了4个可能与长吻生长相关的基因。
In this study,a correlation analysis of 57 single nucleotide polymorphism(SNP)markers and growth-related traits of Leiocassis longirostris was conducted using 115 samples under the same growth conditions.The results showed that 10 loci were related to growth-related traits.Among them,three(cluster-65_137265,cluster-65_111833,and cluster-65_137642)loci had a significant influence on growth-related traits(body weight,total length,body length,and head height)(P<0.05).Cluster-65_120392,cluster-65_105077,and cluster-65_5592_0 had significant influences on body weight,total length,and body length(P<0.05).Cluster-65_110382 significantly influenced total length,body length,and head height(P<0.05).Cluster-65_19497 and cluster-24304_1 had a significant influence on the total length and body length(P<0.05).Cluster-65_130153 significantly influenced body length and head height(P<0.05).We also estimated the genetic diversity parameters for the 10 loci.The mean observed heterozygosity,expected heterozygosity,and the polymorphism information content(PIC)were 0.537,0.467,and 0.357,respectively.In addition,cluster-65_137265,cluster-65_111833,cluster-65_110382,and cluster-65_137642 were associated with anion exchange protein 2-like isoform X1,Netrin-4-like,Tec isoform X2,anion exchange protein 2-like isoform X1,and carbamoyl-phosphate synthase[ammonia]have the highest similarity,respectively.This finding indicates that these four genes may be involved in the regulation of L.longirostris growth.The study provides primary data for the genetic improvement and selective breeding of L.longirostris.
作者
李哲
李雨
敬庭森
刘小莉
闫卉果
陆安帅
周剑
罗辉
叶华
LI Zhe;LI Yu;JING Tingsen;LIU Xiaoli;YAN Huiguo;LU Anshuai;ZHOU Jian;LUO Hui;YE Hua(Fisheries Breeding and Healthy Cultivation Research Centre,Southwest University,Chongqing 402460,China;Fisheries Institute,Sichuan Academy of Agricultural Sciences,Chengdu,Sichuan 611731,China)
出处
《渔业科学进展》
CSCD
北大核心
2022年第4期127-135,共9页
Progress in Fishery Sciences
基金
国家自然科学基金资助项目(31402302)
中央高校基本科研业务费资助项目(XDJK2017B008)
西南大学荣昌校区青年基金资助项目(20700938)共同资助。
关键词
长吻
SNP
生长性状
关联分析
Leiocassis longirostris
SNP markers
Growth traits
Correlation analysis
