摘要
为了比较美国盐酸头孢噻呋、中国大中农盐酸头孢噻呋与中国山东某生物科技公司新研制的盐酸头孢噻呋三种头孢噻呋注射液在白羽鸡血浆中的药物代谢动力学特征,以检测新研制盐酸头孢噻呋注射液在白羽鸡体内的药物动力学参数变化规律。在给白羽鸡肌肉注射药物后,在0至48小时内于不同时间点采集血样,用碘乙酰胺衍生,把二硫赤鲜醇作为提取液,以水-三氟乙酸-乙腈(700∶1∶300)作为流动相,266 nm紫外检测。标准曲线线性相关很好,相关系数都在0995以上。最低检测限为0.05μg/mL;最低定量限为0.1μg/mL,回收率都在90%以上。日间变异系数小于0.1,日内变异系数小于0.05。结果表明,新研制盐酸头孢噻呋的药物动力学参数符合产品要求。三种剂型达峰时间相同;美国盐酸头孢噻呋和新研制盐酸头孢噻呋的半衰期相似,大中农盐酸头孢噻呋半衰期相对要短,但总体三种剂型的半衰期差异不显著;新研制的盐酸头孢噻呋最高血药浓度比美国盐酸头孢噻呋和大中农盐酸头孢噻呋相对高些,美国盐酸头孢噻呋和大中农盐酸头孢噻呋最高血药浓度相当,但总体三种剂型的血药浓度半衰期差异不显著。
Comparing the pharmacokinetic characteristics of three kinds of ceftiofur HCI injected in the plasma of white feather chicken with American Spirit Quick Release ceftiofur,Dazhongnong ceftiofur HCI,and the ceftiofur HCI newly developed by a Shandong,china Biotechnology Co.,Ltd,this paper is aimed to detect whether the pharmacokinetic parameters of Shandong Bio-ceftiofur Hydrochloride Injected in white feather chickens meet the requirements.Blood samples were collected at different time points,and derivatized with iodoacetamide within 0-48 hours,after intramuscular injection of drugs to chicken.Dithioerythritol as the extract solution,water-trifluoroacetic acid-acetonitrile(700∶1∶300)as the mobile phase,UV detection 266 nm.Correlation coefficients are more than 0.995 and the lowest detection limit was 0.05μg/mL;Limit of quantification was 0.1μg/mL,the recovery rate was above 90%.The results showed that the pharmacokinetic parameters of newly developed meet product requirements.The half-lives of Ceftiofur HCl and the newly developed Ceftiofur HCl were similar,while the half-life of Dazhongnong Ceftiofur HCl was relatively shorter,but the overall half-lives of the three dosage forms were not significantly different.
作者
刘坤
王淑婷
田丽华
王玉东
宋翠平
LIU Kun;WANG Shu-ting;TIAN Li-hua;WANG Yu-dong;SONG Cui-ping(China Center for Animal Health And Epidemiology,Laboratory for Quality and Safety Risk Assessment of Livestock and Poultry Products,MARA,Qingdao,Shandong 266032,China;Agricultural and Rural Bureau of Huangdao District,Qingdao,Qingdao,Shandong 266400,China)
出处
《中国兽药杂志》
2022年第7期46-53,共8页
Chinese Journal of Veterinary Drug
基金
中国动物卫生与流行病学中心科技创新基金项目(2021221200)。
