摘要
为了减少料液对层析的干扰,降低层析填料的负荷,提高目标蛋白回收率,文章对两株重组大肠杆菌高密度发酵裂解液澄清工艺进行了研究。选取分别表达猪链球菌和副猪嗜血杆菌关键基因的BL21-HP1036、BL21-palA菌株,采用切向流工艺、高速离心工艺和膜包澄清工艺,通过浊度、电泳检测等方法评估蛋白得率。结果表明:采用膜包过滤清液和膜包浓缩液离心两步法对分别表达猪链球菌和副猪嗜血杆菌关键基因的BL21-HP1036、BL21-palA高密度发酵裂解菌液进行澄清,蛋白得率80%以上,可有效减少层析干扰,提高回收率。
In order to reduce the interference of feed liquid to chromatography,reduce the packing load of chromatography,improve the recovery rate of target protein,this paper studied the clarification process of high-density fermentation lysates of streptococcus suis and Haemophilus parasuis Escherichia coli.The recombinant bacteria BL21-HP1036 and BL21-palA were selected,and the protein yield was evaluated by turbidity,and electrophoresis.The results show that:using membrane filtration clear liquid and membrane concentration liquid centrifugation two-step to study the proteins content of BL21-HP1036 and BL21-palA,the protein yield is more than 80%,which could effectively reduce the chromatography interference and improve the recovery rate.
作者
任培森
吕文静
郑新勇
刘海霞
王艳晓
朱秀同
Ren Peisen;Lyu Wenjing;Zheng Xinyong;Liu Haixia;Wang Yanxiao;Zhu Xiutong(Tianjin Ringpu Biotechnology Co.,Ltd.,Tianjin 300381,China)
出处
《农业技术与装备》
2022年第6期120-122,共3页
Agricultural Technology & Equipment
关键词
重组大肠杆菌
亚单位疫苗
微滤膜包
澄清工艺
recombinant escherichia coli
subunit vaccine
microfiltration membrane package
clarification process
