摘要
目的通过观察IgA肾病模型大鼠小肠黏膜Tfh通络ICOS、CXCR5、Bcl-6、IL-21的变化,探讨补肾健脾-利湿化瘀方对IgA肾病的治疗靶点。方法采用陆慧瑜改良法制备IgA肾病大鼠模型,随机分为空白组、模型组、洛丁新组、补肾健脾-利湿化瘀方(中药高、中、低)剂量组,各15只。给药7周后检测各组小肠黏膜ICOS、CXCR5、Bcl-6、IL-21的表达及肾脏病理的变化。结果与空白组比较,模型组的Tfh相关细胞在小肠黏膜中表达均显著增强(P<0.05),IgA在肾脏沉积明显增多;与模型组大鼠比较,各治疗组Tfh相关细胞在小肠黏膜表达明显减少,IgA在肾脏沉积明显减少,中药中剂量组减少最为显著(P<0.05)。结论补肾健脾-利湿化瘀方通过抑制小肠黏膜生发中心Tfh的过度活化,使IgA浆细胞生成减少,从而缓解肾脏损伤。
Objective To investigate the therapeutic targets of the Bushen Jianpi-Lishi Huayu Recipe on IgA nephropathy by observing the expression changes of ICOS,CXCR5,Bcl-6 and IL-21 in the small intestinal mucosa of IgA nephropathy model rats.Methods The IgA nephropathy rat model was prepared by the modified method of Lu Huiyu,and the model rats were randomly divided into a blank group,a model group,luodingxin group,Bushen Jianpi-Lishi Huayu Recipe groups(high-,medium-and low-dose),with 15 rats in each group.After 7 weeks of administration,the expressions of ICOS,CXCR5,Bcl-6 and IL-21 in the small intestinal mucosa of the rats in each group were detected as well as their changes of renal pathology.Results Compared with the blank group,the expressions of those Tfh-related cells in the small intestinal mucosa of the rats in the model group were all significantly enhanced(P<0.05),and the deposition of IgA in the kidney was significantly increased.Compared with the model group,the expressions of those Tfh-related cells in the small intestinal mucosa and the IgA deposition in the kidney were both significantly decreased in each treatment group,and the decrease was most significant in the TCM medium-dose group(P<0.05).Conclusion The Bushen Jianpi-Lushi Huayu Recipe can reduce the production of IgA plasma cells by inhibiting the excessive activation of Tfh in the germinal center of the small intestine mucosa,thereby alleviating the kidney damage.
作者
王银萍
张洪宝
邹迪
张守琳
WANG Yinping;ZHANG Hongbao;ZOU Di;ZHANG Shoulin(Affiliated Hospital of Changchun University of Chinese Medicine,Changchun 130021,China)
出处
《长春中医药大学学报》
2022年第8期858-861,共4页
Journal of Changchun University of Chinese Medicine
基金
吉林省教育厅“十三五”科学技术项目(JJKH20190465KJ)。
