摘要
探究不同剂量蛹虫草固体发酵物(SSF产物)的安全性和对正常小鼠肠道健康及免疫调节功能的影响,评价SSF产物对机体健康的影响。采用高效液相色谱法分析SSF产物的成分含量。将40只8周龄SPF级BALB/C雄性小鼠随机分为4组:对照组、SSF产物低、中、高剂量[35,70,210 mg/(kg·d)]组,饲养4周后测定小鼠器官的质量、脾淋巴细胞增殖转化能力、血清中细胞因子等指标,采用高通量测序技术检测肠道菌群的变化。结果表明,与子实体相比,SSF产物的蛋白质、粗多糖和虫草素含量分别增加44.8%,92%,146%。不同组小鼠体质量、脏器系数均无显著差异。中、高剂量SSF产物的摄入能降低睾丸脂肪垫系数(P<0.05),并对诱导小鼠细胞免疫调节有显著影响(P<0.05)。SSF产物降低了小鼠肠道菌群多样性,其中条件致病菌丰度显著降低,有益菌群丰度显著上升。SSF产物安全无毒,一定剂量的SSF产物能降低体内脂肪,增强机体免疫功能,降低条件致病菌丰度,促进有益菌的增殖,具有免疫调节功能及促进小鼠肠道健康的作用。
To explore the safety of different doses of Cordyceps militaris solid fermentation(SSF product)and its effects on intestinal health and immunomodulatory function of normal mice,and to evaluate the potential effects of SSF product on body health.The content of solid fermentation products of Cordyceps militariswas analyzed by HPLC.The mice were randomly divided into four groups:control group,SSF product low,middle and high dose[35,70,210 mg/(kg·d)]group.After 4 weeks of feeding,the weight of organs,the proliferation and transformation ability of spleen lymphocytes and the level of cytokines in serum were measured.The changes of intestinal flora were detected by high-throughput sequencing.The results showed that compared with fruiting body,the contents of protein,crude polysaccharide and cordycepin in the solid fermentation of Cordyceps militaris were increased by 44.8%,92%and 146%,respectively.There was no significant difference in body mass and organ coefficient among different groups.The intake of medium and high dose of SSF product could reduce the coefficient of testicular fat pad(P<0.05),and had a significant effect on the induction of cellular immune regulation in mice(P<0.05).The SSF product decreased the diversity of intestinal flora.The abundance of conditional pathogenic bacteria decreased significantly,and the abundance of beneficial bacteria increased significantly.The results showed that the SSF product was safe and non-toxic.A certain dose of SSF product could reduce body fat,enhanced immune function,reduced the abundance of conditional pathogenic bacteria,promoted the proliferation of beneficial bacteria,and had the function of immune regulation and promoting the intestinal health of mice.
作者
陈洁铭
郑涛涛
郭丽琼
林俊芳
郑倩望
Chen Jieming;Zheng Taotao;Guo Liqiong;Lin Junfang;Zheng Qianwang(College of Food Science,South China Agricultural University,Guangzhou 510640;Research Center for Micro-Ecological Agent Engineering and Technology of Guangdong Province,Guangzhou 510642)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2022年第7期76-87,共12页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金项目(32072646,31772373)
广东省科技创新战略专项资金项目(重点领域研发计划)(2018B020205001)
广东省自然科学基金项目(2020A1515010756)。
关键词
蛹虫草
固体发酵
免疫调节
肠道菌群
Cordyceps militaris
solid fermentation
immune regulation
intestinal flora
