摘要
研究β乳球蛋白(β-Lg)与阳离子表面活性剂的相互作用,可以补充β-Lg的基础数据,拓宽其应用范围。文章通过表面张力法、电导率法、圆二色法及内源荧光法考察了3种咪唑类表面活性剂1-烷基-3-甲基溴化咪唑(C_(12)mimBr、C_(14)mimBr及C_(16)mimBr)与β-Lg相互作用过程中的作用方式及表面活性剂对β-Lg结构的影响。结果表明:作用方式与表面活性剂的浓度有关,低浓度的表面活性剂与β-Lg作用方式为静电相互作用,高浓度的表面活性剂与β-Lg作用方式为疏水相互作用;C_(12)mimBr/β-Lg、C_(14)mimBr/β-Lg及C_(16)mimBr/β-Lg体系的反离子结合度分别为0.19、0.28及0.40;低浓度的表面活性剂造成了β-Lg二级结构的改变,但表面活性剂不与β-Lg上的色氨酸残基直接作用。
Studying the interaction betweenβ-lactoglobulin(β-Lg)and cationic surfactants can supplement the basic data ofβ-Lg and broaden its application scope.In this paper,the interaction modes of three imidazole surfactants(C_(12)mimBr,C_(14)mimBr and C_(16)mimBr)withβ-Lg and the effect of the surfactants onβ-Lg structure were investigated by surface tension,electrical conductivity,circular dichroism and internal fluorescence methods.The results show that the interaction mode is related to the concentration of surfactant.The interaction betweenβ-Lg and surfactants in low concentration is electrostatic interaction,while that betweenβ-Lg and surfactants in high concentration is hydrophobic interaction.The anti-ion binding degree of C_(12)mimBr/β-Lg,C_(14)mimBr/β-Lg and C_(16)mimBr/β-Lg systems is 0.19,0.28 and 0.40,respectively.The surfactant in low concentration can cause the change ofβ-Lg secondary structure,but it does not directly interact with the tryptophan residue onβ-Lg.
作者
耿斐
GENG Fei(Editorial Department of Journal of Shandong Jianzhu University,Jinan 250101,China)
出处
《山东建筑大学学报》
2022年第4期108-113,共6页
Journal of Shandong Jianzhu University
基金
山东建筑大学博士科研基金项目(X21073Z)。
