摘要
目的 研究白藜芦醇(RES)对妊娠期至性成熟期亚砷酸钠(NaAsO_(2))暴露子代小鼠发育早期学习记忆和脑氧化应激的影响,探讨砷暴露所致学习记忆受损的可能机制及防治措施。方法 将36只妊娠小鼠分为对照组(12只)及染砷组(24只,饮用含60 mg/L NaAsO_(2)水),其子代小鼠在断乳后至实验结束以饮水方式染砷。仔鼠出生后28 d饮水染砷同时给予RES灌胃干预:来自对照组窝别的仔鼠随机分为对照组和50 mg/kg RES组,来自染砷组的仔鼠随机分为NaAsO_(2)组、NaAsO_(2)+(25、50、100 mg/kg)RES组,每组6只。RES灌胃干预4周,进行水迷宫实验,取各组仔鼠血清和脑组织测定还原型谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)和丙二醛(MDA)水平。结果 从训练第4天起,NaAsO_(2)组仔鼠寻找平台潜伏期显著长于对照组,NaAsO_(2)+RES组仔鼠寻找平台潜伏期显著短于NaAsO_(2)组(P<0.05);空间探索时NaAsO_(2)组仔鼠在目标象限停留时间显著短于对照组,NaAsO_(2)+(25、50 mg/kg)RES组仔鼠在目标象限停留时间显著长于NaAsO_(2)组(P<0.05)。与对照组相比,NaAsO_(2)组仔鼠血清和脑组织中SOD和GSH活性显著下降,LDH和MDA水平明显升高;与NaAsO_(2)组相比,NaAsO_(2)+RES组血清及脑组织中SOD和GSH活性显著升高,LDH和MDA水平显著下降;差异均有统计学意义(P<0.05)。结论 RES可改善砷暴露子代小鼠的氧化应激损伤,从而可能改善砷所致学习记忆能力损伤。
Objective To investigate the effects of resveratrol(RES)on learning,memory and oxidative stress in offspring mice exposed to sodium arsenite(NaAsO_(2))from gestation to sexual maturity,and to explore the possible mechanism of learning and memory impairment caused by arsenic exposure and the measure of prevention and control.Methods A total of 36 Kunming mice from the first day of pregnancy were randomly divided into control group(12 mice)or arsenite exposed group(24 mice,through drinking water),the offspring mice were also exposed to arsenic by drinking water with 60 mg/L of NaAsO_(2) to the end of experiment.On the 28 th day after birth,the offspring mice were given RES by intragastric administration with 25,50 and 100 mg/kg of RES for four weeks,respectively,while the offspring mice in control group were randomly redivided into control and 50 mg/kg RES subgroups,6 mice in each subgroup.Then,Morris water maze was used to determine the effects of arsenic on the learning and memory ability,the levels of superoxide dismutase(SOD),glutathione(GSH),lactate dehydrogenase(LDH)and malondialdehyde(MDA)in serum and brain tissue of mice were measured as well.Results The results showed that from the fourth day of training,the escape latency of mice in NaAsO_(2) group was significantly longer than that of controls,while the escape latency in mice of RES intervention subgroups was significantly less than that of NaAsO_(2) exposed group(P<0.05).In the probe trail,the time spent in target quadrant of arsenic exposed mice was significantly less than that of the control,the time of mice with administration of 25 or 50 mg/kg RES was significantly longer than that of NaAsO_(2) exposed mice(P<0.05).Additionally,compared with the control,the activities of SOD and GSH in serum and brain tissue in arsenic exposed mice decreased significantly,the levels of LDH and MDA increased significantly;while compared with the RES administrated subgroups,the serum and brain activities of SOD and GSH were lower significantly,and the levels of LDH and MDA were higher significantly.Conclusion The results suggested that RES may be helpful to alleviate the oxidative stress injury of mice caused by arsenic exposure,thereby improve the learning and memory impairment caused by arsenic also.
作者
韩晶卓
牛泽华
耿梦尧
王欢
王艳
HAN Jing-zhuo;NIU Ze-hua;GENG Meng-yao;WANG Huan;WANG Yan(Department of Occupational and Environmental Health,School of Public Health,Shenyang Medical College,Shenyang 110034,China)
出处
《中国工业医学杂志》
CAS
2022年第3期212-215,243,共5页
Chinese Journal of Industrial Medicine
基金
国家自然科学基金(81872570)
辽宁省高等学校创新人才支持计划(2020)
辽宁省科学技术计划项目(2021-MS-347)。
