摘要
选择广西南宁市横县和柳州市柳江区15个次生富集型地质高背景土壤,分析土壤的微生物群落结构变化,并从土壤理化性质和重金属Cd全量及有效性等方面分析主要的影响因素。结果发现:(1)研究区土壤全Cd(T-Cd)含量范围在0.214~4.045 mg·kg^(-1),属于Cd地质高背景土壤。EDTA浸提的Cd有效态(Cd-EDTA)含量高于CaCl_(2)浸提的Cd有效态(Cd-CaCl_(2)),且Cd-EDTA与T-Cd含量存在线性关系(R~2=0.66)。(2)研究区土壤细菌群落中最优势种是变形杆菌门(Proteobacteria),其次是绿弯菌门(Chloroflexi),厚壁菌门(Firmicutes)。横县区域土壤变形杆菌门和绿弯菌门相对丰度高于柳江区域土壤;而厚壁菌门相对丰度低于柳江区域土壤。真菌群落中子囊菌门(Ascomycota)是最优势种,其次是担子菌门(Basidiomycota),被孢菌门(Mortierellomycota)。横县区域土壤被孢菌门相对丰度高于柳江区域土壤。(3)研究区土壤细菌丰富度和多样性显著高于真菌,横县区域的细菌丰富度和多样性高于柳江,但真菌的丰富度和多样性低于柳江区域。(4)相关性分析结果表明:土壤pH、SOM和T-Cd与细菌的多样性及丰富度呈负相关,但与真菌的多样性及丰富度呈正相关。真菌多样性和丰富度与Cd-CaCl_(2)呈显著负相关,与Cd-EDTA呈正相关;而细菌多样性和丰富度与Cd-EDTA呈负相关,与Cd-CaCl_(2)呈正相关。
Based on 15 soils with high geological background of secondary enrichment in Hengxian County of Nanning City and Liujiang District of Liuzhou City,Guangxi,we analyzed the variations of microbial community structure and the main influencing factors from the aspects of soil physicochemical properties,total content of heavy metal cadmium(Cd) and its availability.The results showed that soil total Cd(T-Cd) content ranged from 0.214-4.045 mg·kg^(-1),and those soils belonged to high Cd geological background soils.The content of available Cd extracted by EDTA(Cd-EDTA) was higher than that extracted by CaCl_(2)(Cd-CaCl_(2)).There was a linear correlation between Cd-EDTA and T-Cd content(R~2=0.66).The most dominant species of soil bacterial community was from Proteobacteria,followed by Chloroflexi and Firmicutes.The relative abundance of Proteobacteria and Chloroflexi in Hengxian County soils was higher than that in Liujiang soils,while the Firmicutes was lower than that in Liujiang soils.Ascomycota was the most dominant species in the fungal community,followed by Basidiomycota and Mortierellomycota.The relative abundance of Mortierellomycota in Hengxian County soils was higher than that in Liujiang soils.The richness and diversity of soil bacteria in the study area were significantly higher than those of fungi.The richness and diversity of bacteria in Hengxian County soils were higher than those of Liujiang soils,but the richness and diversity of fungi were lower than those in Liujiang soils.Soil pH,SOM,and T-Cd were negatively correlated with the richness and diversity of bacteria,but positively correlated with the richness and diversity of fungi.Fungal diversity and richness had a significant negative correlation with Cd-CaCl_(2),and a positive correlation with Cd-EDTA.Bacterial diversity and richness were negatively correlated with Cd-EDTA,and positively correlated with Cd-CaCl_(2).
作者
代子雯
方成
车婷
李娅娟
胡锋
李辉信
徐莉
DAI Zi-wen;FANG Cheng;CHE Ting;LI Ya-juan;HU Feng;LI Hui-xin;XU Li(College of Resources and Environmental Sciences,Nanjing Agricultural University,Nanjing 210095,China;Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization,Nanjing 210095,China)
出处
《生态学杂志》
CAS
CSCD
北大核心
2022年第8期1493-1500,共8页
Chinese Journal of Ecology
基金
国家重点研发计划项目(2017YFD0800305)
山东省重点研发计划(2019JZZY020614)资助。
关键词
地质高背景土壤
镉
高通量测序
环境因子
土壤微生物群落
high geological background soil
cadmium
high-throughput sequencing
environmental factor
soil microbial community
