摘要
采用组织分离法和单孢分离法对引起川党参根腐病的病原菌进行分离纯化,按照柯赫氏法则对分离物进行致病性验证,并通过形态学观察和真菌r DNA-ITS序列分析鉴定病原菌种类。结果表明,引起重庆市奉节县川党参根腐病的病原菌为腐皮镰孢菌Fusarium solani、燕麦镰孢菌F. avenaceum、锐顶镰孢菌F. acuminatum以及强壮土赤壳菌Ilyonectria robusta。
We used tissue isolation and single spore isolation to isolate and purify the pathog enic fungus causing Codonopsis pilosula subsp. tangshen root rot and verified the pathogenicity of the isolates according to Koch’s postulates.The types of pathogenic fungus were identified by morphological observation and fungal r DNA-ITS sequence analysis. The results showed that Fusarium solani, F. avenaceum, F. acuminatum, and Ilyonectria robusta were the pathogens causing root rot in C. pilosula subsp. tangshen in Fengjie County, Chongqing.
作者
曹剑
覃贵勇
黄志伟
刘露
许彦
游龙
Cao Jian;Qin Guiyong;Huang Zhiwei;Liu Lu;Xu Yan;You Long(Chongqing Three Gorges Vocational College,Wanzhou Chongqing 404155,China)
出处
《中国植保导刊》
北大核心
2022年第6期16-20,共5页
China Plant Protection
基金
重庆市教委科学技术研究项目(KJQN201803502)。
关键词
川党参
根腐病
病原菌
鉴定方法
Codonopsis pilosula subsp.tangshen
root rot
pathogen
identification method
