摘要
【目的】探究不同薄壳山核桃品种的根际土壤真菌群落结构组成、多样性及主要生态功能,分析土壤因子对其影响。【方法】以薄壳山核桃(Carya illinoinensis)波尼(Pawnee)、马罕(Mahan)、金华(Jinhua)3个品种的根际土壤为研究对象,采用高通量测序技术对根际土壤真菌的群落组成、多样性及生态功能进行了分析,并探究了土壤环境与根际土壤真菌群落结构的关系。【结果】金华根际土壤真菌的α多样性显著低于波尼、马罕。与金华相比,波尼和马罕的根际土壤真菌组成结构相似度更高。薄壳山核桃根际土壤真菌群落在门水平上主要由子囊菌门(Ascomycota)、担子菌门(Basidiomycota)及被孢霉菌门(Mortierellomycota)组成;在科水平上以盘菌科(Pezizaceae)和革菌科(Thelephoraceae)为主要优势菌群;属水平上unclassified_f__Pyronemataceae(LDA=5.11、27.01%)、Delastria(LDA=5.44、61.01%)分别在波尼、金华的根际土壤中显著富集,而滑锈伞属(Hebeloma)(LDA=4.64、9.88%)、脆锈伞属(Naucoria)(LDA=4.45、5.67%)则在马罕的根际土壤显著富集(P<0.05)。pH值、铵态氮及有效磷是影响根际土壤真菌群落结构的主要环境因子。FUNGuild功能预测结果表明,根际土壤真菌的功能类型以腐生-共生营养型(42.90%)、共生营养型(38.16%)为主,其次为病理-共生营养型(12.52%)、病理-腐生-共生营养型(4.22%)、腐生营养型(3.17%)和病理营养型(0.57%)。【结论】不同薄壳山核桃品种根际土壤真菌的群落组成及多样性存在显著差异,在属水平上存在具有富集效应的标志菌群。根际土壤真菌在薄壳山核桃的养分循环方面发挥着重要作用。
【Objective】This study aimed to explore the fungal community structure composition,diversity and the main ecological function in rhizosphere soil of different varieties of pecan(Carya illinoinensis),and to reveal their response to the change of soil factors.【Method】The three rhizosphere soils of Pawnee,Mahan and Jinhua of pecan were used as research objects to analyze the community composition,diversity and ecological function of rhizosphere soil fungi through highthroughput sequencing technology.Meanwhile,the relationship between soil factors and rhizosphere soil fungal community structure was also investigated.【Result】Theαdiversity of rhizosphere soil fungi in Jinhua was significantly lower than Pawnee and Mahan.Compared with Jinhua,the community structure of rhizosphere soil fungi in Pawnee and Mahan had a higher similarity.The rhizosphere soil fungi were mainly composed of Ascomycota,Basidiomycota and Mortierellomycota at the phylum level.Pezizaceae and Thelephoraceae were dominant families.Delastria(LDA=5.44,61.01%)and unclassified_f_Pyronemataceae(LDA=5.11,27.01%)were obviously enriched in the rhizosphere of Jinhua and Pawnee respectively,while Hebeloma(LDA=4.64,9.88%)and Naucoria(LDA=4.45,5.67%)were enriched in Mahan at the genus level(P<0.05).The pH value,ammonium nitrogen and available phosphorus were the main factors affecting rhizosphere fungal community structure.Based on the functional prediction of FUNGuild,saprotroph-symbiotroph(42.90%)and symbiotroph(38.1%)were the main functional types of the rhizosphere soil fungi,followed by the pathotrophsymbiotroph(12.52%),pathotroph-saprotroph-symbiotroph(4.22%),saprotroph(3.17%)and pathology(0.57%).【Conclusion】The rhizosphere soil fungal community composition and diversity differed significantly among different varieties of pecan and there were biomarkers with obvious enrichment effect at the genus level.Meanwhile,rhizosphere soil fungi played an important role in nutrient utilization and cycling during the growth of pecan plantation.
作者
汤玉洁
刘俊萍
储国林
刘帅
彭方仁
TANG Yujie;LIU Junping;CHU Guolin;LIU Shuai;PENG Fangren(College of Forestry,Nanjing Forestry University,Nanjing 210037,China;Co-Innovation Center for the Sustainable Forestry in Southern China,Nanjing 210037,China)
出处
《河南农业大学学报》
CAS
CSCD
2022年第4期622-633,共12页
Journal of Henan Agricultural University
基金
国家重点研发专项项目(2018YFD1000604)
江苏高校优势学科建设工程资助项目(PAPD)。
关键词
薄壳山核桃
根际土壤真菌
高通量测序
功能预测
Carya illinoinensis
rhizosphere soil fungi
high-throughput sequencing
functional prediction
