摘要
为了探讨铁皮石斛提取物在化妆品中的应用,通过清除ABTS自由基试验、酪氨酸酶活性抑制试验、封闭性斑贴测试、临床功效测试,验证铁皮石斛提取物的效果。结果显示,铁皮石斛提取物对ABTS自由基有较好的清除效果,半数清除浓度值(IC_(50))为(6.84±0.94)mg/mL。对酪氨酸酶具有较好的抑制作用,IC_(50)为(8.57±0.81)mg/mL。根据封闭性斑贴测试,显示含质量分数10%铁皮石斛提取物的精华液无刺激性。32名受试者使用6周后结果表明,与使用前相比皮肤含水量增长15.34%、经皮失水减少17.84%、皮肤黄度减少5.46%、透亮度增加5.67%,均有显著改善(P<0.01)。因此,铁皮石斛提取物具有较强抗氧化和抑制酪氨酸酶功效,温和安全,长期使用含质量分数10%铁皮石斛提取物的精华液能达到保湿、保护皮肤屏障、祛黄、美白功效,值得临床推广和使用。
In order to explore the application of Dendrobium candidum extract in cosmetics,the effect of Dendrobium candidum extract was verified by ABTS free radical scavenging experiment,tyrosinase activity inhibition experiment,patch test and clinical efficacy test.The results showed that Dendrobium candidum extract had a good scavenging effect on ABTS free radicals,with IC_(50) of(6.84±0.94)mg/mL.It has a good inhibitory effect on tyrosinase,with IC_(50) of(8.57±0.81)mg/mL.The serum containing 10%(mass fraction)Dendrobium candidum extract was shown to be non-irritating according to the patch test.After 6 weeks of treatment,the results of 32 subjects showed that the skin water content increased by 15.34%,the transdermal water loss decreased by 17.84%,the skin yellowness decreased by 5.46%,and the transparency increased by 5.67%,all of which were significantly improved(P<0.01).Thus,Dendrobium candidum extract has strong antioxidation and tyrosinase inhibition effects.Long-term use of the essence containing 10%(mass fraction)Dendrobium candidum extract can achieve moisturizing,protecting skin barrier,whitening effect,worthy of clinical promotion and use.
作者
招敏聪
刘文
芦晓红
刘裕娇
贺锐
ZHAO Mincong;LIU Wen;LU Xiaohong;LIU Yujiao;HE Rui(Dr.Plant(Guangdong)Biotechnology Co.,Ltd.,Foshan 528399,Guangdong,China;Beijing Dr.Plant Biotechnology Co.,Ltd.,Beijing 100055,China)
出处
《香料香精化妆品》
CAS
2022年第4期94-99,共6页
Flavour Fragrance Cosmetics
关键词
铁皮石斛提取物
抗氧化
酪氨酸酶
临床功效
Dendrobium candidum extract
antioxidant
tyrosinase
clinical efficacy
