摘要
目的 研究千金子提取物在肝癌MHCC97-H细胞增殖、迁移、侵袭和凋亡中的作用与潜在分子机制。方法 用千金子提取物5 mg/mL、10 mg/mL、20 mg/mL分别处理肝癌细胞MHCC97-H,CCK-8法和流式细胞术检测MHCC97-H细胞增殖和凋亡,Transwell实验检测细胞迁移和侵袭能力,Western blot检测Cyclin D1、p21、Bax、Bcl-2、E-cadherin和MMP-2蛋白表达水平,qRT-PCR检测lncRNA AK125512的转录水平。结果 千金子提取物可抑制MHCC97-H细胞增殖、迁移和侵袭,促进细胞凋亡,提高lncRNA AK125512的水平,降低Cyclin D1、Bcl-2和MMP-2的表达(P<0.05),促进p21、Bax和E-cadherin的表达;过表达AK125512可抑制MHCC97-H细胞增殖、迁移和侵袭,促进细胞凋亡;抑制AK125512可逆转千金子提取物对MHCC97-H细胞增殖、迁移、侵袭和凋亡的作用。结论 千金子提取物通过提高AK125512水平抑制MHCC97-H细胞的增殖、迁移和侵袭,促进细胞凋亡。千金子提取物对肝癌具有潜在治疗作用。
Objective To explore the effects of Semen Euphorbiae Lathyris extract on proliferation, migration, invasion and apoptosis of hepatocellular carcinoma MHCC97-H cells and the potential mechanism.Method MHCC97-H cells were treated with 5 mg/mL, 10 mg/mL and 20 mg/mL of Semen Euphorbiae Lathyris extract respectively. The proliferation and apoptosis of MHCC97-H cells were detected by CCK-8 and flow cytometry. The migration and invasion abilities of MHCC97-H cells were determined by Transwell assay. The proteins of Cyclin D1, p21, Bax, Bcl-2, E-cadherin and MMP-2 protein levels were detected by Western blot. The transcriptional level of lncRNA AK125512 in MHCC97-H cells was measured by qRT-PCR.Result The Semen Euphorbiae Lathyris extract inhibited the proliferation, migration and invasion of MHCC97-H cells, promoted cell apoptosis, increased the level of lncRNA AK125512(P<0.05), decreased the expression of Cyclin D1, Bcl-2 and MMP-2(P<0.05), and promoted the expression of p21, Bax and E-cadherin(P<0.05). Overexpression of AK125512 inhibited the proliferation, migration and invasion of MHCC97-H cells and promoted cell apoptosis. Inhibition of AK125512 reversed the effects of Semen Euphorbiae Lathyris extract on proliferation, migration, invasion and apoptosis of MHCC97-H cells.Conclusion The Semen Euphorbiae Lathyris extract inhibits the proliferation, migration and invasion of MHCC97-H cells and promotes cell apoptosis by increasing the level of AK125512. The Semen Euphorbiae Lathyris extract has potential therapeutic effects on hepatocellular carcinoma.
作者
贺丽娜
刘力
贺晓旭
王建钧
何松
HE Li-na;LIU Li;HE Xiao-xu(Department of Hepatobiliary Surgery,Affili-ated Hospital of Xiangnan University,Chenzhou 423000,China)
出处
《肝胆外科杂志》
2022年第3期228-232,239,共6页
Journal of Hepatobiliary Surgery
关键词
千金子提取物
肝癌
长链非编码RNA
AK125512
增殖
迁移
侵袭
凋亡
semen euphorbiae lathyris extract
hepatocellular carcinoma
long non-coding RNA AK125512
proliferation
migration
invasion
apoptosis
