摘要
目的了解医院不同科室病房空气中耐药菌的污染情况。方法于2018年11月至2021年6月采集武汉市3家医院部分科室病房空调回风口滤网积尘样本191份,对积尘中的耐药菌进行筛选、鉴定,选择19种常用抗生素对检出的金黄色葡萄球菌、鲍曼不动杆菌、肠杆菌科细菌等目标菌进行药敏试验。结果119份积尘样本检出耐药菌,阳性率为62.30%,共筛选、鉴定出362株耐药菌,主要为不动杆菌属(28.73%)、芽胞杆菌属(24.31%)、假单胞菌属(22.10%)、葡萄球菌属(9.12%)等,不同科室积尘样本的阳性率由高到低依次为外科(68.29%)>重症监护室(59.62%)>内科(57.97%);其中检出72株目标菌,检出率为19.89%(72/362),不同目标菌的检出率由高至低依次为鲍曼不动杆菌(12.71%)>肠杆菌科细菌(4.42%)>金黄色葡萄球菌(2.76%)(P<0.05)。72株目标菌的药敏试验结果显示,41株为耐药菌,检出率为56.94%(41/72),包括耐碳青霉烯类鲍曼不动杆菌、耐甲氧西林金黄色葡萄球菌、耐碳青霉烯类肠杆菌科细菌等,其中24株为多重耐药菌,检出率为58.54%(24/41),不同科室多重耐药菌的检出率由高到低依次为重症监护室(80.00%)>内科(60.00%)>外科(46.15%)。结论武汉市3家医院部分科室空气环境中存在与医院感染相关的鲍曼不动杆菌、肠杆菌科细菌与金黄色葡萄球菌等耐药菌的污染,其中有些为多重耐药菌,主要分布于神经内科、呼吸内科、甲乳外科、神经外科、心胸外科病房以及胆胰重症监护室、综合重症监护室。
OBJECTIVE To investigate the contamination of antibiotic-resistant bacteria in air of different departments in hospital.METHODS From 2018.07 to 2021.06,191 samples of the air-conditioning filter dust in three hospitals were collected.Antibiotic-resistant bacteria were isolated from the accumulated dust.The drug sensitivity test was conducted for Staphylococcus aureus,Acinetobacter baumannii and Enterobacteriaceae.RESULTS A total of 119 samples were detected antibiotic-resistant bacteria from 191 samples,and the detection rate was 62.30%.The detection rate of different departments from high to low was surgical ward(68.29%)>intensive care unit(ICU)(59.62%)>medical ward(57.92%).A total of 362 strains of antimicribial-resistant organisms were isolated,mainly were Acinetobacter(28.73%),Pseudomonas(22.10%),Bacillus(22.10%),Staphylococcus(9.12%),etc.Among them,72 strains of target organisms were detected,and the detection rate was 19.89%(72/362),the detection rate of different target bacteria from high to low was Acinetobacter baumannii(12.71%)>Enterobacteriaceae(4.72%)>Staphylococcus aureus(2.76%)(P<0.05).The drug sensitivity test showed that 41 strains of antimicribial-resistant organisms were detected,and the detection rate was 56.94%(41/72),including carbapenem-resistant Acinetobacter baumannii(CR-ABA),methicillin-resistant Staphylococcus aureus(MRSA),carbapenem-resistant Enterobacteriaceae(CRE),etc.24 strains of multidrug-resistant organisms(MDROs)were detected and the detection rate was 58.54%(24/41).The detection rate of different departments from high to low was ICU(80.00%)>medical ward(60.00%)>surgical ward(46.15%).CONCLUSION There was contaminated by Acinetobacter baumannii,Staphylococcus aureus,Enterobacteriaceae in the air of hospitals,some of them were MDROs,mainly were detected in neurological ward,respiratory medical ward,hyroid and breast surgery ward,neurosurgery ward,cardiothoracic surgery ward,gallideulous surgical ICU and general ICU.
作者
邱婉月
夏雨荷
龚林
袁凤云
陈琪
李家豪
梁建生
唐非
Qiu Wanyue;Xia Yuhe;Gong Lin;Yuan Fengyun;Chen Qi;Li Jiahao;Liang Jiansheng;Tang Fei(Institute of Environmental Medicine,MOE Key Laboratory of Environment and Health,School of Public Health,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Hubei Provincial Center for Disease Prevention and Control,Wuhan 430079,China;Department of Disinfection and Pest Control,Wuhan Centers for Disease Prevention and Control,Wuhan 430015,China;Jiang’an District Center for Disease Prevention and Control,Wuhan 430010,China)
出处
《卫生研究》
CAS
CSCD
北大核心
2022年第4期617-623,共7页
Journal of Hygiene Research
关键词
医院
空调过滤网
积尘
空气
耐药菌
hospital
air-conditioning filter
dust
air
antibiotic-resistant bacteria
