摘要
目的研究富含丝氨酸和精氨酸的剪接因子6(serine and arginine rich splicing factor 6,SRSF6,又称SRp55)作为RNA剪切因子在大鼠胰腺β细胞中对胰岛素分泌影响的具体机制。方法通过改进的RNA结合蛋白免疫共沉淀高通量测序(improved RNA Binding Protein Immunoprecipitation highthroughput sequencing,iRIP-seq)技术在大鼠胰岛细胞中鉴定SRSF6调控的基因及功能通路,对SRSF6直接作用的靶标和调控通路及可变剪接事件进行鉴定。结果SRSF6富集性地结合编码区和内含子区域,KEGG分析发现SRSF6结合的靶标在胰岛素信号通路显著富集。结论SRSF6可能通过与胰岛素通路相关重要基因的m RNA前体结合,调控它们的剪接和加工,进而成为影响胰岛素分泌的潜在因素。
Objective This study aimed to investigate the specific mechanism of the effect of RNA splicing factor SRSF6(SRp55)on insulin secretion in rat pancreaticβcells.Methods In this study,the genes and functional pathways regulated by SRSF6were identified in rat islet cells by iRIP-seq high-throughput sequencing,and the targets,regulatory pathways and alternative splicing events directly affected by SRSF6 were identified.Results SRSF6 enriched CDS and Intron regions,and KEGG analysis showed that SRSF6-bound targets were significantly enriched in insulin signaling pathway.Conclusion SRSF6 may be a potential factor affecting insulin secretion by binding to pre-mRNAs of important genes related to insulin pathway and regulating their splicing and processing.
作者
马艳荣
罗蕴之
马福慧
周忠凯
Ma Yanrong;Luo Yunzhi;Ma Fuhui(Department of Endocrinology and Metabolism,People’s Hospital of Xinjiang Uygur Autonomous Region,Urumgi 830001,China)
出处
《中华保健医学杂志》
2022年第4期336-338,共3页
Chinese Journal of Health Care and Medicine
基金
新疆维吾尔自治区自然科学基金项目(2021D01C169)。
