摘要
野菊(Chrysanthemum indicum)是重要的资源植物,广泛分布于东北、华北、华中、华南和西南地区,具有较高的药用和观赏价值。bZIP转录因子参与许多种植物的生物学过程,对植物抵抗逆境胁迫起着较高的调控作用。利用生物信息学分析的方法在野菊全基因组数据库中对野菊bZIP转录因子进行全基因组鉴定,预测和分析基因结构、蛋白性质、蛋白结构、启动子。结果表明,野菊bZIP转录因子家族共有75个基因;亲缘关系较近的野菊bZIP基因的基因结构较为相似;保守基序分析结果表明,每个基因所包含的保守基序的种类、数量及分布位置具有一定相似性,但也存在差异;聚在同一亚家族的基因所含有的保守基序的种类、数量和分布位置相似。bZIP转录因子家族基因的启动子中有多种顺式作用元件,其中光反应性元件最多,其次是植物激素反应性元件。野菊bZIP转录因子家族的分析结果可为进一步研究野菊bZIP基因的功能和分子机制提供了依据。
Chrysanthemum indicum is an important resource plant,widely distributed in Northeast,North,Central,South and Southwest China,and has high medicinal and ornamental value.bZIP transcription factor participates in many kinds of plant biology processes,and plays a higher regulatory role in plant resistance to adversity stress.Through the whole genome database of wild chrysanthemum,using bioinformatics analysis method,the whole genome identification of wild chrysanthemum bZIP transcription factor was carried out.Through the prediction and analysis of gene composition,physical and chemical properties of protein,protein model structure,cis-acting elements and expression of promoter level.The results showed that the wild chrysanthemum bZIP transcription factor family has a total of 75 genes;the genetic structure of the wild chrysanthemum bZIP genes with close relationship is relatively similar;The results of conserved motif analysis showed that the types,numbers and distribution positions of conserved motifs contained in each gene have certain similarities,but there are also differences;The conserved motifs contained in genes clustered in the same subfamily are similar in type,number and distribution.There are a variety of cis-acting elements in the promoters of bZIP transcription factor family genes,of which photo-responsive elements are the most,followed by plant hormone-responsive elements.The analysis results of the wild chrysanthemum bZIP transcription factor family can lay a theoretical foundation for further research on the function and molecular mechanism of the wild chrysanthemum bZIP gene.
作者
刘贺
黄沁梅
刘颖婕
何淼
周蕴薇
Liu He;Huang Qinmei;Liu Yingjie;He Miao;Zhou Yunwei(Cllge of Landieape Archietur,Norheat Foreatry University,Harbin,150040;College of Hortiealure,lin Agriultural University,Changchun,130118)
出处
《分子植物育种》
CAS
北大核心
2022年第14期4586-4600,共15页
Molecular Plant Breeding
基金
国家自然科学基金项目(31870687)资助。
