摘要
目的探讨玉郎伞多糖(YLSPS)对脂多糖(LPS)诱导的人甲状腺滤泡上皮细胞炎症损伤和凋亡的影响及作用机制。方法不同浓度的YLSPS处理LPS诱导的人甲状腺滤泡上皮细胞Nthy-ori3-1后,转染Caveolin-1过表达载体至Nthy-ori3-1细胞;酶联免疫吸附法检测白细胞介素1β(IL-1β)、白细胞介素6(IL-6)和单核细胞趋化蛋白1(MCP-1)水平,流式细胞仪检测细胞凋亡,实时荧光定量PCR(RT-qPCR)检测Caveolin-1 mRNA表达水平,蛋白印迹(Western Blot)法检测Caveolin-1蛋白表达。YLSPS处理甲状腺炎模型小鼠,显微镜观察各组小鼠甲状腺组织形态变化。结果YLSPS可降低LPS诱导的Nthy-ori3-1细胞中IL-1β、IL-6和MCP-1水平、细胞凋亡率(P<0.05),促进细胞中Caveolin-1的mRNA及蛋白表达水平(P<0.05),且具有剂量依赖性。过表达Caveolin-1也可降低LPS诱导的Nthy-ori3-1细胞中IL-1β、IL-6和MCP-1水平及细胞凋亡率。YLSPS可改善甲状腺炎模型小鼠甲状腺组织形态,降低小鼠血清中IL-1β、IL-6和MCP-1水平,促进甲状腺组织中Caveolin-1蛋白表达。结论YLSPS可抑制LPS诱导的Nthy-ori3-1细胞炎症损伤和凋亡,其作用机制与上调Caveolin-1表达有关。
Objective To investigate the effect and action mechanism of Yutaro polysaccharide(YLSPS)on lipopolysaccharide(LPS)-induced inflammatory injury and apoptosis of human thyroid follicular epithelial cells.Methods After being treated with different concentrations of YLSPS,the LPS-induced human thyroid follicular epithelial cells Nthy-ori3-1 were transfected with the Caveolin-1 overexpressed vectors.The levels of interleukin 1(IL-1),interleukin 6(IL-6),and monocyte(MCP-1)chemotactic protein 1 were measured by enzyme-linked immunosorbent assay,and apoptosis was measured by flow cytometry.The Caveolin-1 mRNA expression levels were measured by quantitative real-time PCR(RT-qPCR)and Caveolin-1 protein expression was determined by protein blotting(Western Blot).The thyroiditis model mice were treated with YLSPS and the thyroid tissues in each group were observed in view of morphologybymicroscopy.Results YLSPS reduced the levels of IL-1,IL-6and MCP-1 and apoptosis(P<0.05)in the LPS-induced cells,and promoted the mRNA and protein expression levels of Caveolin-1(P<0.05)in the cells in a dose-dependent manner.Overexpressed Caveolin-1 also decreased the levels of IL-1,IL-6,and MCP-1 and the rate of apoptosis in the Nthy-ori3-1 cells.YLSPS improved the thyroid tissue in morphology,reduced IL-1,IL-6,and MCP-1 levels in the mouse serum,and promoted Caveolin-1 protein expression in the thyroid tissues.Conclusion YLSPS can inhibit the inflammatory damage and apoptosis in Nthy-ori3-1 cells,and its mechanism of action is related to the upregulation of Caveolin-1 expression.
作者
辛思源
杨志新
柳金英
刘慧
郭建恩
XIN Siyuan;YANG Zhixin;LIU Jinying;LIU Hui;GUO Ji-an′en(Chengde Medical College,Chengde 067000,China)
出处
《实用医学杂志》
CAS
北大核心
2022年第14期1759-1765,1772,共8页
The Journal of Practical Medicine
基金
河北省自然科学基金项目(编号:H2021406054)
河北省高等学校科学技术研究项目(编号:QN2020201)
河北省高等教育教学改革研究与实践项目(编号:2020GJJG595)。
