摘要
目的分析基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)检测支气管肺泡灌洗液(BALF)对涂阴或无痰肺结核(PTB)的诊断价值。方法以2020年1月至2021年12月安徽省胸科医院收治的87例疑似涂阴或无痰PTB患者为研究对象,对其BALF进行MALDI-TOF MS、SAT-TB、Gene-Xpert及BD960法检测,并对其诊断价值进行比较。结果MALDI-TOF MS诊断涂阴或无痰PTB敏感性为68.5%(37/54),优于SAT-TB 13.0%(7/54)、Gene-Xpert 40.7%(22/54)、BD960法27.8%(15/54)(均P<0.01)。MALDITOF MS法AUC值为0.827,优于SAT-TB 0.550、Gene-Xpert 0.704、BD960法0.639(均P<0.01)。MALDITOF MS分别联合上述3种方法的AUC并不优于其单独检测。结论MALDI-TOF MS是较准确的辅助诊断涂阴或无痰PTB的新方法,有较好的应用前景。
Objective To investigate the efficacy of matrix assisted laser desorption/Ionization-time of flight mass spectrometry(MALDI-TOF MS)for the diagnosis of smear-negative or sputum-scarce pulmonary tuberculosis(PTB)by testing bronchoalveolar lavage fluid(BALF).Methods BALF of 87 patients with suspected smear-negative or sputum-scarce tuberculosis were tested by MALDI-TOF MS,SAT-TB,Gene-Xpert and BD960from January 2020 to December 2021 in Anhui Chest Hospital.The results from those methods were compared.Results The sensitivity of MALDI-TOF MS for diagnosing smear-negative or sputum-scarce PTB was 68.5%(37/54),which was better than that of SAT-TB 13.0%(7/54),or Gene-Xpert 40.7%(22/54),or BD96027.8%(15/54)(all P<0.01).The AUC value of MALDI-TOF MS was 0.827,which was better than that of SAT-TB 0.550,or Gene-Xpert 0.704,or BD9600.639(all P<0.01).Combining MALDI-TOF MS with any one of the other three methods did not produce a better AUC value than using it alone.Conclusion MALDI-TOF MS appears to be a more accurate method in assisting the diagnosis of smear-negative or sputum-scarce PTB.
作者
侯婧
王华
刘盛盛
HOU Jing;WANG Hua;LIU Shengsheng(Department of Tuberculosis,Anhui Pro-vincial Chest Hospital,Hefei 230022,China)
出处
《实用医学杂志》
CAS
北大核心
2022年第13期1599-1603,共5页
The Journal of Practical Medicine
基金
国家“十三五”传染病科技重大专项“耐药肺结核中医药治疗新方案研究”(编号:2018ZX10725-509)。
关键词
基质辅助激光解吸电离飞行时间质谱
结核
支气管肺泡灌洗液
诊断
matrix assisted laser desorption/ionization-time of flight mass spectrometry
tuberculosis
bronchoalveolar lavage fluid
diagnosis
