摘要
目的探讨鼠李糖乳杆菌及姜黄素对代谢相关脂肪性肝病(MAFLD)模型大鼠干预的作用及可能的机制。方法将40只SD雄性大鼠适应性喂养1周后随机分为正常对照组(MCS)、模型对照组(MCD)、鼠李糖乳杆菌干预组(LGG)、姜黄素干预组(CUR)和鼠李糖乳杆菌联合姜黄素干预组(LGG+CUR),每组8只;实验第4周末处死大鼠,比较5组大鼠肝指数、肝组织病理变化及脂肪含量,检测血清转氨酶、炎性因子水平以及肝组织TLR4、NF-κB和MyD88的表达情况。结果MCD组与MCS组比较,一般情况差,肝脏组织大面积脂肪变性及脂质浸润,血清转氨酶、炎性因子明显升高,证实了蛋氨酸胆碱缺乏饮食喂养4周成功复制了大鼠MAFLD模型;各干预组大鼠肝指数、肝脏病理变化和肝脏脂肪水平,血清转氨酶、炎性因子水平,TLR4、NF-κBp65和MyD88的表达均较MCD组显著降低,LGG+CUR组降低最为显著,差异有统计学意义(P<0.01)。结论鼠李糖乳杆菌和姜黄素均能明显改善MAFLD大鼠肝脏脂质沉积、肝功能,降低肝指数及炎性因子的表达,可能与调节TLR4信号通路,降低肝中TLR4、MyD88和NF-κB的表达有关。
Objective To investigate the effects of Lactobacillus rhamnosus and curcumin on liver pathological changes,liver index,liver function,serum inflammatory factors and liver tissue TLR4,MyD88,NF-κB expression in MAFLD model rats and its possible mechanism.Methods After 1 week of adaptive feeding,40 SD male rats were randomly divided into normal control group(MCS),model control group(MCD),Lactobacillus rhamnoides intervention group(LGG),curcumin intervention group(CUR)and Lactobacillus rhamnoides plus curcumin intervention group(LGG+CUR)with 8 rats in each group.At the end of the 4th week,the rats were killed,and the liver index,pathological changes and fat content of liver tissue were compared,and the levels of serum transaminase,inflammatory factors and the expression of TLR4,NF-κBp65 and MyD88 in liver tissue were detected.Results Compared with MCS group,the general condition of MCD group was worse,large area steatosis and lipid infiltration of liver tissue,serum transaminase and inflammatory factors were significantly increased,which confirmed that the rat model of MAFLD was successfully established by methionine choline deficiency diet for 4 weeks.The liver index,liver pathological changes,liver fat level,serum transaminase,inflammatory factors,and the expression of TLR4,NF-κBp65 and MyD88 in each intervention group were significantly lower than those in MCD group,and the decrease was the most significant in LGG+CUR group.Conclusion Both Lactobacillus rhamnosus and curcumin can significantly improve liver lipid deposition,liver function and reduce the expression of liver index and inflammatory factors in MAFLD rats,which may be related to the regulation of TLR4 signal pathway and the decrease of the expression of TLR4,MyD88 and NF-κB in liver.
作者
徐子媛
张九娜
孟灿灿
程燕
郭永泽
李校天
XU Ziyuan;ZHANG Jiu′na;MENG Cancan;CHENG Yan;GUO Yongze;LI Xiaotian(Clinical Medical College of Hebei University of Engineering,Handan 056000,China)
出处
《实用医学杂志》
CAS
北大核心
2022年第13期1624-1629,共6页
The Journal of Practical Medicine
