摘要
目的探讨复方苦参注射液对人膀胱癌细胞株BIU-87细胞增殖及裸鼠膀胱原位移植瘤生长的影响。方法将对数生长期BIU-87细胞分为实验组(分别加入300.00、150.00、75.00、37.50、18.75µl/ml复方苦参注射液200µl)和阴性对照组(加入等量培养液),四甲基偶氮唑盐(MTT)法检测并计算BIU-87细胞的增殖抑制率及半数抑制浓度(IC_(50))。20只BALB/c-nu雌性裸鼠膀胱灌注100μl细胞密度2×10^(7)/ml的BIU-87细胞悬液,建立膀胱原位移植瘤动物模型;灌注BIU-87细胞悬液24 h后开始膀胱腔内灌注给药(100μl/只),采用随机数字表法分为复方苦参注射液组(膀胱灌注苦参原液)、吡柔比星组(膀胱灌注1 mg/ml吡柔比星)、模型对照组(膀胱灌注等量的灭菌注射用水)、空白对照组(膀胱不灌注BIU-87细胞悬液且不给药);观察时间为90 d,观察并记录动物存活状况、膀胱湿重,计算成瘤率、肿瘤抑制率、生命延长率。结果不同浓度复方苦参注射液作用BIU-87细胞48 h,300.00、150.00、75.00、37.50、18.75μl/ml复方苦参注射液组和阴性对照组吸光度(A)值分别为0.027±0.006、0.065±0.010、1.695±0.105、2.387±0.017、2.427±0.134和2.721±0.080(F=742.67,P<0.05),各浓度复方苦参注射液组A值与阴性对照组比较,差异均有统计学意义(均P<0.05)。复方苦参注射液对BIU-87细胞的IC50值为70.05µl/ml。观察90 d时,空白对照组、模型对照组、吡柔比星组、复方苦参注射液组裸鼠膀胱湿重分别为(0.018±0.004)mg、(0.422±0.130)mg、(0.219±0.136)mg、(0.237±0.113)mg(F=14.01,P<0.001),裸鼠存活时间分别为(90±0)d、(54±12)d、(72±4)d、(69±8)d(F=18.53,P<0.001)。吡柔比星组和复方苦参注射液组对膀胱癌的抑制率分别为48.10%和43.84%,对裸鼠的生命延长率分别为34.95%和29.53%。结论复方苦参注射液可抑制BIU-87细胞增殖并具有浓度依赖性;复方苦参注射液可提高裸鼠膀胱原位移植瘤动物模型抑瘤率,延长裸鼠存活时间。
Objective To investigate the effects of compound matrine injection on the proliferation of bladder cancer cell line BIU-87 and bladder orthotopic transplantated tumor in nude mice.Methods BIU-87 cells in logarithmic growth phase were divided into experimental group(adding 300.00,150.00,75.00,37.50,18.75μl/ml compound matrine injection 200μl)and negative control group(adding equal volume of culturing medium).The proliferation inhibition rate and the half inhibitory concentration(IC_(50))of BIU-87 cells were detected and calculated by methyl thiazole tetrazolium(MTT)method.Twenty BALB/c-nu female nude mice were injected with 100μl of BIU-87 cell suspension with a cell density of 2×10^(7)/ml in the bladder to establish an animal model of bladder orthotopic transplanted tumor.After 24 hours of perfusion of BIU-87 cell suspension,intravesical perfusion administration(100μl per nude mouse)was started,and the mice were divided into compound matrine injection group(intravesical perfusion of matrine solution)and pirarubicin group(intravesical perfusion of 1 mg/ml pirarubicin),model control group(intravesical perfusion of the same volume of sterile water),blank control group(without intravesical perfusion of BIU-87 cell suspension or administration).The observation time was 90 d.The survival status and bladder wet weight of the animals were observed and recorded,and the tumor formation rate,tumor inhibition rate and life prolongation rate were calculated.Results Different concentrations of compound matrine injection acted on BIU-87 cells for 48 hours,and the absorbance(A)values of 300.00,150.00,75.00,37.50,18.75μl/ml compound matrine injection group and negative control group were 0.027±0.006,0.065±0.010,1.695±0.105,2.387±0.017,2.427±0.134 and 2.721±0.080(F=742.67,P<0.05),the A values of each concentration of compound matrine injection group were compared with the negative control group,and the differences were statistically significant(all P<0.05).The IC_(50) of compound matrine injection on BIU-87 cells was 70.05μl/ml.On the 90th day of observation,the bladder wet weights of nude mice in blank control group,model control group,pirarubicin group and compound matrine injection group were(0.018±0.004)mg,(0.422±0.130)mg,(0.219±0.136)mg and(0.237±0.113)mg(F=14.01,P<0.001),and the survival time of nude mice was(90±0)d,(54±12)d,(72±4)d and(69±8)d(F=18.53,P<0.001).The inhibition rates of bladder cancer in the pirarubicin group and compound matrine injection group were 48.10%and 43.84%,and the life prolongation rates of the nude mice were 34.95%and 29.53%.Conclusions Compound matrine injection can inhibit the proliferation of BIU-87 cells in a concentration-dependent manner.Compound matrine injection can increase the tumor inhibition rate and prolong the survival time of nude mice models of bladder orthotopic transplanted tumor.
作者
郭晋锋
魏锦萍
张红兵
李佳
Guo Jinfeng;Wei Jinping;Zhang Hongbing;Li Jia(Dean's Office,Shanxi Province Cancer Hospital,Shanxi Hospital Affiliated to Cancer Hospital,Chinese Academy of Medical Sciences,Cancer Hospital Affiliated to Shanxi Medical University,Taiyuan 030013,China;Radiotoxicology Room,Institute of Radiology and Environmental Medicine,China Institute for Radiation Protection,Taiyuan 030006,China;Department of Quality,Huawei Pharmaceutical Co.,Ltd.,China Institute for Radiation Protection,Taiyuan 030006,China;Department of Abdominal and Pelvic Radiotherapy 1,Shanxi Province Cancer Hospital,Shanxi Hospital Affiliated to Cancer Hospital,Chinese Academy of Medical Sciences,Cancer Hospital Affiliated to Shanxi Medical University,Taiyuan 030013,China)
出处
《肿瘤研究与临床》
CAS
2022年第7期498-502,共5页
Cancer Research and Clinic
关键词
膀胱肿瘤
细胞增殖
原位移植瘤
复方苦参注射液
膀胱灌注
Urinary bladder neoplasms
Cell proliferation
Orthotopic transplanted tumor
Compound matrine injection
Intravesical perfusion
