摘要
目的 观察WE43镁合金骨植入对大鼠血常规、肝肾功能指标、全血金属元素、脏器组织学的影响,以及植入体的体内降解情况。方法 将12只大鼠随机分成对照组和镁合金植入组,每组6只。将WE43镁合金加工后植入镁合金植入组大鼠的双侧股骨髁,对照组不做任何处理,相同条件下饲养6个月。比较两组大鼠血常规、肝肾功能指标及全血金属元素(镁、锌、钇、钕、锆)含量;取两组大鼠肝脏、肾脏和脾脏组织,观察组织病理学变化;采用Micro CT扫描检测镁合金植入组大鼠左侧股骨髁植入物的降解情况和植入物周围成骨情况。结果 骨植入6个月后,两组大鼠血常规、肝肾功能指标、镁和锌含量比较,差异均无统计学意义(均P>0.05),且两组大鼠全血中均未检出钇、钕、锆元素。两组大鼠肝、肾和脾组织未见明显异常变化。Micro CT扫描结果显示,WE43镁合金植入物较植入前减少约25%的体积,植入物周围有新生骨形成。结论 WE43镁合金骨植入6个月后可出现明显的体内降解,其对大鼠血常规、全血金属元素、肝肾功能,以及肝、肾和脾组织形态的影响均较小。
Objective To observe the influence of WE43 magnesium alloy bone implantation on blood routine, liver and kidney function indicators, whole blood metallic elements, and organ histology of rats, as well as the in vivo degradation.Methods A total of 12 rats were randomly assigned to control group or magnesium alloy implantation group, with 6 rats in each group. After WE43 magnesium alloy processing, it was implanted in rats′ bilateral femoral condyles in the magnesium alloy implantation group, while the control group did not undergo any processing. The rats were fed for 6 months under the same conditions. The blood routine, liver and kidney function indices, and whole blood metallic elements(magnesium, zinc, yttrium, neodymium, and zirconium) contents were compared between the two groups. The tissues of rats′ liver, kidney and spleen in both groups were obtained, and the histopathological changes were observed. The Micro CT scan was used to detect the degradation and surrounding osteogenesis of the implants in rats′ left femoral condyles of the magnesium alloy implantation group.Results After 6 months of bone implantation, there were no statistically significant differences in blood routine, liver and kidney function indices, and the contents of magnesium and zinc between the two groups(all P>0.05), and no element of yttrium, neodymium and zirconium in the whole blood of the rats was detected in both groups. There were no obvious abnormalities in the liver, kidney and spleen tissues in the rats between the two groups. The Micro CT scan results revealed that the volume of WE43 magnesium alloy implants decreased by approximately 25% compared with before implantation, and there was neonatal bone formation around the implants.Conclusion WE43 magnesium alloy can present significant in vivo degradation at 6 months after bone implantation, exerting slight influence on blood routine, whole blood metallic elements, liver and kidney functions, as well as the histomorphology of liver, kidney and spleen tissues.
作者
张亚男
肖鹏
孙瑞
罗亚鸽
谢文
冯坤
ZHANG Ya-nan;XIAO Peng;SUN Rui;LUO Ya-ge;XIE Wen;Feng Kun(Faculty of Acupuncture Massage and Rehabilitation,Hunan University of Chinese Medicine,Changsha 410208,Hunan,China;Medical Laboratory Centre,Luoyang Orthopedic-Traumatological Hospital of Henan Province,Luoyang 471002,Henan,China;Henan Institute of Osteopathy,Henan Engineering Research Center of Orthopedic Medical Devices,Luoyang 471002,Henan,China)
出处
《广西医学》
CAS
2022年第14期1624-1628,1635,共6页
Guangxi Medical Journal
基金
河南省科技攻关计划(162102310366)。
关键词
镁合金
骨植入
体内降解
血常规
肝肾功能
全血元素
脏器组织
大鼠
Magnesium alloy
Bone implantation
In vivo degradation
Blood routine
Liver and kidney function
Whole blood elements
Organ tissues
Rat
