摘要
为了解稻瘟病抗性与根际土壤微生物之间的关系,在云南宜良大田条件下对6个稻瘟病单基因系Piz、Pib、Pikh、Pi19、Pi3、Pita和受体“丽江新团黑谷”(LTH)进行了诱发鉴定,利用ITS1和16SrDNA高通量测序技术对土壤根际真菌的ITS1序列以及细菌16S rDNA区片段进行了分析。结果显示单基因系Piz、Pib、Pikh表现抗病, Pi19、Pi3、Pita和LTH表现感病;子囊菌门(Ascomycota)是真菌的优势门(73.41%),变形菌门(Proteobacteria)是细菌的优势门(32.48%),7个土样在主要微生物种类组成上相似;抗、感单基因系根际土壤真菌在罗兹菌门(Rozellomycota)(分别为7.69%和14.66%, LTH为5.59%)及其下属的罗兹菌种(Rozellomycota_sp)(分别为6.97%和13.99%, LTH为5.59%)和担子菌门(Basidiomycota)的伞菌纲(Agaricomycetes)(分别为1.2%和0.7%,LTH为1.7%)及伞菌目(Agaricale)(分别为1.1%和0.5%,LTH为1.6%)上存在显著性差异(P<0.05);细菌在变形菌门(Proteobacteria)的γ-变形菌纲未鉴定的目上存在显著性差异(P<0.05),相对平均丰度分别为10.02%和11.65%,受体LTH为8.55%,表明水稻抗性对根际土壤微生物丰度有影响。结果为探究稻瘟病的抗病机理和生物防治提供了线索。
To understand the relationship between the rhizosphere microbes and resistance to rice blast, six monogenic lines include genes Piz, Pib, Pikh, Pi19, Pi3, Pita to rice blast and receptor "Lijiangxintuanheigu"(LTH) were cultivated in Yiliang of Yunnan Province and were induced by appraisal. ITS1 sequence of soil rhizosphere fungi and V3-V4 sequence in 16S rDNA of bacteria were sequenced by using high throughput sequencing technology.The results showed that the monogenic lines Piz, Pib and Pikh showed resistance to blast, but Pi19, Pi3, Pita and LTH showed susceptibility. Microbial species query analysis suggest that Ascomycota was the dominant phylum of fungi(73.41%), Proteobacteria was the dominant phylum of bacteria(32.48%), and 7 soil samples were similar in composition of main microbial groups. The relative abundance with significant differences in Rozellomycota(7.69% and 14.66%, LTH5.59%), Rozellomycota_sp(6.97%and 13.99%, LTH5.59%), Agaricomycetes(1.2% and 0.7%, LTH1.7%)and Agaricale(1.1% and 0.5%, LTH1.6%) between resistant and susceptible monogenic lines rhizosphere soil fungi(P<0.05). The relative abundance of Proteobacteria-proteobacteria belongs to bacterial was 10.02% and 11.65% and the receptor LTH was 8.55%, indicated that rice resistance had an influence on the abundance of rhizosphere soil microbial. The results of this study provide a clue to explore the mechanism of disease resistance and biological control of rice blast.
作者
张翠
孙一丁
马继琼
杨奕
郭怡卿
李进斌
许明辉
ZHANG Cui;SUN Yiding;MA Jiqiong;YANG Yi;GUO Yiqing;LI Jinbin;XU Minghui(Key Laboratory of Biotechnology Research of Yunnan Province,Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation of Ministry of Agriculture,Kunming 650205,China;College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;Institute of Biotechnology and Genetic Resources,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;Institute of Agricultural Environment and Resources,Yunnan Academy of Agricultural Sciences,Kunming 650205,China)
出处
《生态科学》
CSCD
2022年第6期130-138,共9页
Ecological Science
基金
国家自然科学基金项目(31860369)。
