摘要
目的探讨血清微RNA(miR)-29a-3p、弹性蛋白(ELN)在早产儿支气管肺发育不良(BPD)患儿中的表达意义。方法选取2019年1月至2021年7月该院131例早产儿为研究对象,分为BPD组(34例)和非BPD组(97例)。采用实时荧光定量PCR(qRT-PCR)法检测miR-29a-3p水平,ELISA法检测早产儿出生后第1、7、14天血清ELN水平,并进行相关性、受试者工作特征(ROC)曲线及多因素分析。结果与非BPD组比较,BPD组机械通气时间、吸氧时间更长,最高氧浓度、院内感染率更高,差异有统计学意义(P<0.05)。早产儿出生后第7、14天,BPD组miR-29a-3p相对表达水平高于非BPD组,ELN水平低于非BPD组,差异有统计学意义(P<0.05)。随着时间推移,BPD组miR-29a-3p相对表达水平逐渐上升,ELN水平逐渐下降,不同时间点比较差异有统计学意义(P<0.05)。BPD组早产儿出生后第7天miR-29a-3p与ELN水平呈负相关(r=-0.571,P<0.05)。早产儿出生后第7天miR-29a-3p、ELN及其联合预测BPD发生的曲线下面积(AUC)分别为0.863、0.878、0.949。miR-29a-3p(OR=2.379,95%CI:1.582~3.576)、ELN(OR=0.837,95%CI:0.765~0.916)是早产儿BPD发生的独立影响因素(P<0.05)。结论血清miR-29a-3p、ELN水平检测对BPD有早期预测价值。
Objective To investigate the expression significance of microRNA(miR)-29a-3p and elastin(ELN)in premature infants with bronchopulmonary dysplasia(BPD).Methods A total of 131 premature infants in our hospital were divided into 34 cases in BPD group and 97 cases in non-BPD group.Real-time fluorescent quantitative PCR(qRT-PCR)and enzyme-linked immunosorbent assay(ELISA)method was used to detect miR-29a-3p and ELN on the 1st,7th,and 14th day of premature infants,and correlation,receiver operating characteristic(ROC)curve and multi factor analysis were carried out.Results Compared with the non-BPD group,the mechanical ventilation time and oxygen inhalation time of BPD group were longer,the highest oxygen concentration and nosocomial infection rate were higher,and the difference was statistically significant(P<0.05).On the 7th and 14th day after birth,the relative expression level of miR-29a-3p in BPD group was higher than that in non-BPD group,and ELN level was lower than that in non-BPD group,the difference was statistically significant(P<0.05).With the passage of time,the serum miR-29a-3p level in BPD group gradually increased,and ELN level gradually decreased,and the difference was statistically significant at different time points(P<0.05).Serum miR-29a-3p and ELN levels of premature infants in BPD group were negatively correlated on the 7th day after birth(r=-0.571,P<0.05).The area under the curve(AUC)of serum miR-29a-3p,ELN and their combination predicting the occurrence of BPD in preterm infants on the 7th day after birth was 0.863 and 0.878,0.949,respectivel y.MiR-29a-3p(OR=2.379,95%CI:1.582-3.576)and ELN(OR=0.837,95%CI:0.765-0.916)were independent factors influencing the occurrence of BPD in premature infants(P<0.05).Conclusion The detection of serum miR-29a-3p and ELN levels has early predictive value for BPD.
作者
许花芬
冯琼
李春蕾
XU Huafen;FENG Qiong;LI Chunlei(Department of Neonatology,Hainan Genernal Hospital,Hainan Affiliated Hospital of Hainan Medical University,Haikou,Hainan 570311,China)
出处
《重庆医学》
CAS
2022年第17期2932-2935,2941,共5页
Chongqing medicine
基金
海南省卫生计生行业科研项目(1801320114A2026)。
