摘要
为挖掘具有缓解植烟土壤酸化能力的微生物菌株,采用盆栽试验和大田试验相结合的方法,应用生物形态学和分子生物学筛选鉴定了γ-聚谷氨酸(γ-PGA)高产菌株并研究其对酸化植烟土壤的改良效果。结果表明:(1)从黄土高原干旱土壤中筛选得到一株谷氨酸非依赖型γ-PGA高产菌株副地衣芽孢杆菌285-3(Bacillus paralicheniformis);(2)菌株285-3可有效缓解根系分泌物和复合肥料导致的土壤酸化,土壤pH平均提高0.3个单位以上;(3)菌株285-3配施有机肥,可显著降低酸化植烟土壤可交换酸度,对烟草青枯病的防效达到47.05%,同时提高烟叶产质量。综上所述,副地衣芽孢杆菌285-3对酸化植烟土壤有较好的改良效果,与有机肥合用具有控病和提高烟叶产质量的作用。
In order to explore the microbial strains with the ability to alleviate the acidification of tobacco planting soil,a combination of biomorphology and molecular biology,pot experiment and field experiment were used to screen and identify high-yieldingγ-PGA strains and study their improvement effects on acidified tobacco planting soil.The results showed that:(1)Aglutamate-independentγ-PGA producing strain Bacillus paralicheniformis 285-3 was screened from the arid soil of the Loess Plateau.(2)Strain 285-3 effectively alleviated soil acidification caused by root exudates and compound fertilizers,and increased soil pH by more than 0.3 units on average.(3)The combination of strain 285-3 and organic fertilizer significantly reduced the exchangeable acidity of acidified tobacco planting soil,and the control effect of tobacco bacterial wilt reached 47.05%,while improved the yield and quality of tobacco.In conclusion,Bacillus paralicheniformis 285-3 has a good improvement effect on acidified tobacco plating soil,and can control disease and improve yield and quality of tobacco when combined with organic fertilizer.
作者
施河丽
彭五星
向修志
舒照鹤
向必坤
王雪松
上官力
尹忠春
祁高富
谭军
SHI Heli;PENG Wuxing;XIANG Xiuzhi;SHU Zhaohe;XIANG Bikun;WANG Xuesong;SHANG Guanli;YIN Zhongchun;QI Gaofu;TAN Jun(Enshi Prefecture Company of Hubei Provincial Tobacco Corporation,Enshi,Hubei 445000,China;College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)
出处
《中国烟草科学》
CSCD
北大核心
2022年第4期15-21,共7页
Chinese Tobacco Science
基金
中国烟草总公司湖北省公司科技项目(027Y2019-011)。
关键词
酸化植烟土壤
副地衣芽孢杆菌
Γ-聚谷氨酸
烟草青枯病
烟叶产质量
acidified tobacco planting soil
Bacillus paralicheniformis
γ-PGA
tobacco bacterial wilt
tobacco yield and quality
