摘要
为比较循环水系统中各水处理单元细菌群落组成、物种多样性和功能的差异,通过高通量测序技术分析该系统养殖池、沉淀池、蛋白分离池、生物滤池和海马齿浮床池水体中的菌群组成,并进行菌群功能预测。结果显示,与其他池相比,生物滤池和海马齿浮床池中α-变形菌纲显著减少,拟杆菌门明显增高。疣微菌门在生物滤池中的相对丰度显著高于其他池。从属的水平分析,优势菌Loktanella在沉淀池和蛋白分离池的占比是其他池的2~3倍,Rubritalea在生物滤池的占比明显高于其他池。海马齿浮床池菌群丰度和菌群多样性均最高,生物滤池菌群丰度最低。在群落组成和结构方面,生物滤池和海马齿浮床池与其他样品相似度最低。PICRUSt功能预测结果显示,该循环水系统中细菌群落占优势的功能类群主要集中在新陈代谢方面。比较各单元细菌群落功能相似性发现,沉淀池和蛋白分离池较相似,生物滤池则与海马齿浮床池类似。另外氮代谢功能基因的相对丰度在海马齿浮床池中更高。该试验结果可为构建适合于中国南方地区的循环水养殖系统提供数据支撑和技术支持。
In order to compare the differences in community composition, species diversity and function of bacteria in the recirculating aquaculture systems(RAS), bacterial species composition and predicted function in the water in fish production tanks, sedimentation filter, protein skimmer, biofilter tank and Sesuvium portulacastrum floating bed tank were analyzed by high-throughput sequencing technology. The results showed that compared with other tanks, the relative abundance of Alphaproteobacteria in biofilter and floating bed tanks were significantly reduced while Bacteroidetes markedly increased, with significantly higher Verrucomicrobia in biofilter tank. According to the genus level analysis, the proportion of dominant bacterium Loktanella in sedimentation filter and protein skimmer was 2—3 times as that in other tanks, meanwhile Rubritalea in biofilter tank was significantly higher than others, with the maximal species richness and diversity in floating bed tank, and the minimal flora abundance in the biofilter tank. There was the minimal similarity with other samples in terms of community composition and structure in the biofilter and floating bed tank. The PICRUSt functional prediction results showed that the dominant functional groups of bacterial communities in the RAS were mainly in the aspect of metabolism. Comparing the functional similarity of bacterial communities in each unit, it was found that the sedimentation tank and protein separation tank were similar, while the biofilter was similar to the floating bed tank. In addition, the relative abundance of nitrogen cycle function genes in floating bed tank was obviously higher. The findings can provide data and technical support for the construction of the RAS suitable for southern China.
作者
熊向英
王志成
刘旭佳
姚坤志
梁志辉
XIONG Xiangying;WANG Zhicheng;LIU Xujia;YAO Kunzhi;LIANG Zhihui(Guangxi Institute of Oceanology Limited Liability Company,Beihai 536000,China;Guangxi Key Laboratory of Marine Environmental Science,Guangxi Beibu Gulf Marine Research Center,Guangxi Academy of Sciences,Nanning 530007,China)
出处
《水产科学》
CAS
CSCD
北大核心
2022年第5期810-819,共10页
Fisheries Science
基金
广西科技计划项目(AB16380167,AB18221112)
广西科学院基本科研业务费资助项目(2019YJJ1004)
北海市科技计划项目(201884028)。
关键词
循环水养殖
细菌群落
高通量测序
recirculating aquaculture system
bacterial community
high-throughput sequencing
