摘要
B型烟粉虱Bemisia tabaci是重大入侵害虫,发展生物防治技术控制其危害具有重要意义。本研究采用浸渍法测定了9种20株土壤真菌对B型烟粉虱的室内活性,结果表明,供试菌株对B型烟粉虱都有一定致病活性,1.0±108孢子/mL的分生孢子悬浮液处理10 d后,烟粉虱2龄若虫的累计校正死亡率为53%~93%,其中淡紫紫孢菌Purpureocillium lilacinum PITS01、PlMS-12和PlMS-15菌株,以及刀孢蜡蚧菌Lecanicillium psalliotae LpTS01菌株引起B型烟粉虱91%以上的死亡率。进一步测定了PITS01和LpTS01菌株对烟粉虱的毒力,结果表明PITS01和LpTS01对烟粉虱2龄若虫在处理第4天的LC_(50)分别为2.8×10^(7)孢子/mL和3.6×10^(7)孢子/mL,第6天分别为1.7×10^(6)孢子/mL和3.6×10^(5)孢子/mL;当孢子浓度为1.0×10^(7)孢子/mL时,PITS01和LpTS01菌株LT_(50)值分别为4.4 d和4.2 d。菌株PITS01和LpTS01对烟粉虱防治具有一定的应用潜力。
The B-biotype of whitefly(Bemisia tabaci)is an important invasion pest.It is significant to develop biocontrol technology to overcome the pest damage.In current study,the bioactivities of 20 fungal strains in 9 species from soil against whitefly nymph were evaluated by means of immersion method.The results indicated that all the strains had certain pathogenicity to B.tabaci.After treated with 1.0×10^(8)spores/mL for 10 days,the calibrated mortality of the 2^(nd) instar nymphs of B.tabaci was 53%~93%.Among them,the three strains of Purpureocillium lilacinum PITS01,BbTs03,AuTS01 and the Lecanicillium psalliotae LpTS01 strain had higher bioactivity with whiteflies’mortality>91%.Furthermore,the experiments of virulence tests indicated that the LC^(50) of PITS01 and LpTS01 to the 2^(nd) instar nymph of B.tabaci were 2.8×10^(7)spores/mL and 3.6×10^(7)spores/mL on the 4^(th) day after treatment,and 1.7×10^(6)spores/mL and 3.6×10^(5)spores/mL on the 6^(th) day after treatment,respectively.Meanwhile,the LT_(50) values of PITS01 and LpTS01 at 1.0×10^(7)spores/mL of the concentration,were respectively recorded as 4.4 d and 4.2 d.Obviously,the results suggested that the strains PITS01 and LpTS01 had the potential for whitefly biocontrol.
作者
古开平
吴伟
胡琼波
GU Kai-Ping;WU Wei;HU Qiong-Bo(College of Plant Protection,South China Agricultural University,Guangzhou 510642,China;Zhejiang Ecopro Agro Technology Company,Ltd.,Jiashan 314199,Zhejiang Province,China)
出处
《环境昆虫学报》
CSCD
北大核心
2022年第4期1010-1018,共9页
Journal of Environmental Entomology
基金
广州市科技计划项目(201903010067)。
关键词
土壤真菌
淡紫紫孢菌
刀孢蜡蚧菌
烟粉虱
Soil fungi
Purpureocillium lilacinum
Lecanicillium psalliotae
Bemisia tabaci
bioactivity
