摘要
目的探究LncRNA FOXD3-AS1在卵巢癌SKOV3细胞中的表达及影响SKOV3细胞恶性生物学行为的作用机制。方法收集2020年10月~2021年6月我院病理科经过临床确诊的55例卵巢癌患者,利用RT-qPCR技术分析卵巢癌组织、癌旁组织、SKOV3细胞和IOSE80细胞中FOXD3-AS1、miR-325和CDCA5的表达;siRNA靶向敲降FOXD3-AS1和CDCA5的表达,通过细胞克隆形成实验、细胞迁移实验和Western blot检测SKOV3细胞增殖、迁移及其EMT能力。miRanda、TargetScan和双荧光素酶报告基因实验分析LncRNA FOXD3-AS1与miR-325、miR-325与CDCA5之间的关系。miR-325 inhibitor转染细胞,或pcDNA-FOXD3-AS1与miR-325 mimics共转染细胞后分析SKOV3细胞增殖、迁移和EMT情况。结果与IOSE80细胞、癌旁组织对比,SKOV3细胞、卵巢癌组织内LncRNA FOXD3-AS1、CDCA5表达上调(P<0.01),miR-325表达下调(P<0.01)。siRNA靶向敲降FOXD3-AS1和CDCA5的表达抑制了SKOV3细胞增殖、迁移与EMT;LncRNA FOXD3-AS1的下游作用靶点为miR-325,miR-325的作用靶点为CDCA5;pcDNA-FOXD3-AS1与miR-325 mimics共转染细胞后部分逆转了miR-325 mimics转染细胞对SKOV3细胞增殖、迁移和EMT的抑制作用。结论LncRNA FOXD3-AS1在卵巢癌SKOV3细胞中表达上调及通过miR-325/CDCA5轴影响SKOV3细胞恶性生物学行为。
Objective To explore the expression of LncRNA FOXD3-AS1 in ovarian cancer SKOV3 cells and the mechanism of its influence on the malignant biological behavior of SKOV3 cells.Methods Fifty-five patients with ovarian cancer diagnosed clinically in the department of pathology of our hospital from October 2020 to June 2021 were collected.The expression of FOXD3-AS1,miR-325 and CDCA5 in ovarian cancer tissues,paracancer tissues,SKOV3 cells and IOSE80 cells were analyzed by RT-qPCR.The expression of FOXD3-AS1 and CDCA5 was knockdown by siRNA,the proliferation,migration and EMT of SKOV3 cells were detected by cell cloning formation assay,cell migration assay and Western blot.The relationships between LncRNA FOXD3-AS1 and miR-325,miR-325 and CDCA5 were analyzed by miRanda,TargetScan and dual luciferin reporter assay.The proliferation,migration and EMT of SKOV3 cells were analyzed after transfection with miR-325 inhibitor or co-transfection with pcDNA-FoxD3-AS1 and miR-325 mimics.Results Compared with IOSE80 cells and paracancer tissues,the expression of LncRNA FOXD3-AS1 and CDCA5 in SKOV3 cells and ovarian cancer tissues was up-regulated(P<0.01),and the expression of miR-325 was down-regulated(P<0.01),siRNA knockdown of FOXD3-AS1 and CDCA5 inhibited SKOV3 cell proliferation,migration and EMT.The downstream target of LncRNA FOXD3-AS1 is miR-325,and the target of miR-325 is CDCA5.After co-transfection with miR-325 mimics,pcDNA-FOXD3-AS1 partially reversed the inhibitory effect of miR-325 mimics transfected cells on proliferation,migration and EMT of SKOV3 cells.Conclusion LncRNA FOXD3-AS1 expression was up-regulated in ovarian cancer SKOV3 cells and affected the malignant biological behavior of SKOV3 cells through miR-325/CDCA5 axis.
作者
黄丽
龚豪
张春莲
黄润强
任松森
HUANG Li;GONG Hao;ZHANG Chunlian;HUANG Runqiang;REN Songsen(Department of Gynecology,Shiyan Taihe Hospital,The Affiliated Hospital of Hubei Medical College,Shiyan 442000,Hubei,China;Department of Nephrology,Shiyan Taihe Hospital,The Affiliated Hospital of Hubei Medical College,Shiyan 442000,Hubei,China)
出处
《西部医学》
2022年第9期1288-1295,共8页
Medical Journal of West China
基金
湖北省卫生健康科研立项项目(WJ2019Q015)。
