摘要
【目的】试验旨在研究多形性腺瘤基因1(plemorphic adenoma gene 1,PLAG1)基因序列特征、多态性以及对山羊出生体重、体尺的影响。【方法】以波尔山羊为研究对象,克隆PLAG1基因序列并测序,采用实时荧光定量PCR鉴定其在不同年龄和体重波尔山羊组织中的表达模式,采用Western blotting方法检测PLAG1在不同体重羔羊腿肌中的表达水平,进一步筛选PLAG1基因CDS和3′-UTR区SNP,并分析各位点不同基因型与波尔山羊出生体重、体尺的相关性。【结果】波尔山羊PLAG1基因CDS全长1 500 bp,编码499个氨基酸,PLAG1蛋白相对保守。组织表达谱显示,PLAG1基因在出生体重较大的羔羊心脏、小肠和腿肌中表达水平显著或极显著高于出生体重较小的羔羊,在胎羊各组织中mRNA表达水平均显著或极显著高于成年母羊(P<0.05;P<0.01);PLAG1蛋白在出生体重较大的羔羊腿肌中表达量极显著高于出生体重较小的羔羊(P<0.01)。在波尔山羊PLAG1基因3′-UTR区共筛选到6个SNPs位点,分别为:2664 A>G、2712 A>G、2721 T>C、2879 T>A、2990 A>T和3270 A>G。关联分析发现,2664 A>G位点AG基因型个体出生管围显著大于GG基因型(P<0.05);2721 T>C位点TT基因型个体出生体长显著大于TC基因型(P<0.05);2879 T>A位点TA基因型个体出生管围显著大于AA基因型(P<0.05);2990 A>T位点AA基因型个体出生体重显著高于AT基因型(P<0.05);3270 A>G位点GG基因型个体出生体长显著大于AA基因型,AG基因型个体出生胸围显著大于AA基因型(P<0.05)。【结论】PLAG1基因在波尔山羊胎羊各组织中表达水平均高于成年母羊,发育早期的表达水平与出生羔羊体重相关。PLAG1基因可作为分子标记用于波尔山羊早期生长选育。
【Objective】 This study was aimed to investigate the sequence characteristics, polymorphisms of plemorphic adenoma gene 1(PLAG1) gene and its effects on birth weight and body size in goats.【Method】 Boer goats were selected as the object, the PLAG1 gene were cloned and sequenced, the expression pattern of PLAG1 gene in various tissues of Boer goats at different ages and birth weight was identified by Real-time quantitative PCR,the PLAG1 protein level of leg muscle in lambs with different birth weight was detected by Western blotting.The SNPs in CDS and 3′-UTR of PLAG1 gene in Boer goats were further screened, and its correlation between different genotypes and birth weight and body size in Boer goats were analyzed.【Result】 The total length of PLAG1 gene CDS in Boer goats was 1 500 bp, encoding 499 amino acids.PLAG1 protein was relatively conservative.The tissue expression profile showed that the expression of PLAG1 gene in heart, small intestine and leg muscle in lambs with large birth weight were significant or extremely significantly higher than those with small birth weight, the expression of different tissues in fetal Boer goats was significantly or extremely significantly higher than that in adult ewes(P<0.05 or P<0.01). The expression of PLAG1 protein in leg muscles of lambs with large birth weight was extremely significantly higher than those with small birth weight(P<0.01).6 SNPs of PLAG1 gene 3′-UTR in Boer goats were screened, which were 2664 A>G,2712 A>G,2721 T>C,2879 T>A,2990 A>T and 3270 A>G,respectively.Correlation analysis showed that the birth tube circumference of AG genotype individuals at 2664 A>G was significantly longer than that of GG genotype(P<0.05);The birth length of TT genotype individuals at 2721 T>C was significantly longer than that of TC genotype(P<0.05);The birth tube circumference of TA genotype individuals at 2879 T>A was significantly longer than that of AA genotype(P<0.05);The birth weight of AA genotype individuals at 2990 A>T was significantly higher than that of AT genotype(P<0.05);The birth length of GG genotype individuals at 3270 A>G was significantly longer than that of AA genotype, and the birth chest circumference of AG genotype individuals at 3270 A>G was significantly larger than that of AA genotype(P<0.05).【Conclusion】 The expression of PLAG1 gene in different tissues of fetal goats were higher than those of adult ewes, the expression during early development was related to the birth weight.PLAG1 gene could be used as a molecular marker for early growth and breeding of Boer goats.
作者
冯小品
李隐侠
张晨俭
张俊
孟春花
钱勇
曹少先
FENG Xiaopin;LI Yinxia;ZHANG Chenjian;ZHANG Jun;MENG Chunhua;QIAN Yong;CAO Shaoxian(Institute of Animal Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Jiangsu Agricultural Germplasm Resources Protection and Utilization Platform,Nanjing 210014,China;Key Laboratory of Planting and Breeding,Ministry of Agriculture and Rural Affairs,Nanjing 210014,China)
出处
《中国畜牧兽医》
CAS
北大核心
2022年第9期3465-3474,共10页
China Animal Husbandry & Veterinary Medicine
基金
江苏省农业重大新品种创制项目(PZCZ201740)。
