摘要
目的 制备沙棘益生菌微胶囊,并进行耐酸性和肠溶性研究。方法 以海藻酸钠、乳清分离蛋白和氯化钙的混合物为壁材,采用微胶囊技术将沙棘益生菌发酵液进行包埋,制备得到沙棘益生菌微胶囊。结果 选取半乳糖增殖培养基作为益生菌的活化培养基,半乳糖浓度为2.000%时,对益生菌的增殖效果最佳,不仅缩短了益生菌的延滞期,而且提高益生菌的生长速率;经过150min的模拟胃液处理后,仍然具有较高的存活率,为61%;在模拟肠液处理中处理60min之后,益生菌就会被连续的释放出来。结论 沙棘益生菌微胶囊在胃液中具有良好耐酸性,在肠液中具有良好的肠溶性。
Objective To prepare the microcapsules of Hippophae rhamnoides Linn.probiotics,and study the acid resistance and enteric solubility.Methods Using a mixture of sodium alginate,whey protein isolate and calcium chloride as the wall materials,the fermentation broth of Hippophae rhamnoides Linn.probiotics was embedded by microencapsulation technology,and the Hippophae rhamnoides Linn.probiotic microcapsules were prepared.Result Galactose proliferation medium was selected as the activation medium of probiotics.When the concentration of galactose was 2.000%,the proliferation effect of probiotics was the best,which not only shortened the lag period of probiotics,but also increased the growth rate of probiotics.After 150 min of simulated gastric juice treatment,it still had a high survival rate,and the survival rate was 61%.After 60 minutes of treatment in the simulated intestinal fluid treatment,the probiotics were continuously released.Conclusion Hippophae rhamnoides Linn.probiotics microcapsules have good acid resistance in gastric juice and good enteric solubility in intestinal fluid.
作者
周莉
朱海华
谭静
平洋
张亚勋
王法云
ZHOU Li;ZHU Hai-Hua;TAN Jing;PING Yang;ZHANG Ya-Xun;WANG Fa-Yun(The Institute of Commercial Sciences Co.,Ltd.,of Henan Province,Zhengzhou 450002,China)
出处
《食品安全质量检测学报》
CAS
北大核心
2022年第16期5171-5177,共7页
Journal of Food Safety and Quality
基金
河南省科学院杰青人才培养项目(210411003)
河南省省级特聘研究员项目(200511003)。
关键词
沙棘
益生菌
微胶囊
耐酸性
肠溶性
Hippophae rhamnoides Linn.
probiotics
microcapsules
acid resistance
enteric solubility
