摘要
为提高山楂叶多酚提取效率,验证山楂叶多酚降糖活性,以山楂叶为原料,采用微波预处理和超声波辅助提取多酚,利用Box-Behnken设计优化山楂叶多酚提取工艺条件,探讨山楂叶多酚对α-葡萄糖苷酶和α-淀粉酶抑制作用,评价多酚体外降糖效果;采用STZ建立糖尿病小鼠模型,经不同剂量山楂叶多酚提取物灌胃连续4周后测定空腹血糖值、胰岛素浓度、肝糖原含量、GHb、肝脏和胰腺指数、肝脏ALT和AST活性,研究山楂叶多酚体内降糖效果。结果表明,山楂叶多酚最佳提取条件为乙醇浓度70%、液料比401 mL·g^(-1)、超声时间25 min、超声温度56℃,多酚得率0.75%;山楂叶多酚对α-葡萄糖苷酶和α-淀粉酶均有较好抑制作用,当山楂叶多酚浓度为4.5 mg·mL^(-1)时,抑制率分别为82.8%和88.5%;山楂叶多酚有效降低糖尿病小鼠空腹血糖、血清胰岛素水平、GHb含量、肝脏指数及ALT和AST活性,提高小鼠肝糖原含量、胰腺指数,呈剂量依赖,高剂量(600 mg·kg^(-1))山楂叶多酚效果最佳,说明山楂叶多酚具有较好体内降糖能力。上述研究结果可为山楂叶多酚高效制备及降糖功能食品开发提供理论依据。
In order to improve the extraction efficiency of hawthorn leaf polyphenols and verify its hypoglycemic activity,polyphenols were extracted from hawthorn leaves which were used as raw materials by using the microwave pretreatment and ultrasonic assisted methods,the extraction process of hawthorn leaf polyphenols were optimized by the Box Behnken experimental design,the inhibition effects of hawthorn leaf polyphenols onα-glucosidase andα-amylase were explored,the hypoglycemic effect of in vivo polyphenols was evaluated,and the hypoglycemic effect of hawthorn leaf in vivo polyphenols was researched by applying STZ to establish the model of diabetes mice in which the fasting blood glucose,insulin concentration,GHb,liver glycogen content,liver and pancreas index,liver ALT and AST activities were measured after intragastric administration of different doses of hawthorn leaf polyphenols extractions for four weeks.The results showed that the optimum extraction conditions of polyphenols from hawthorn leaves were as the following:ethanol concentration was 70%,liquid material ratio was 40:1 mL·g^(-1),ultrasonic time was 25 min,ultrasonic temperature was 56℃,and the yield of polyphenols was 0.75%.The hawthorn leaf polyphenols inhibitedα-glucosidase andα-amylase effectively,the inhibition rates were respectively 82.8%and 88.5%when the concentration of polyphenols in hawthorn leaves was 4.5 mg·mL^(-1).The hawthorn leaf polyphenols could effectively reduce fasting blood glucose,serum insulin level,GHb content,liver index,ALT and AST activities in diabetes mice,and increase liver glycogen content and pancreatic index in a dose-dependent manner,and the effect of hawthorn leaf polyphenols with high dose(600 mg·kg^(-1))was the best,indicated that hawthorn leaf polyphenols had a good hypoglycemic ability in vivo.The above results provided a theoretical basis for the efficient preparation of hawthorn leaf polyphenols and the development of hypoglycemic functional food.
作者
陈旭
卢静静
王涵驰
李延华
CHEN Xu;LU Jingjing;WANG Hanchi;LI Yanhua(College of Food&Biology Science and Technology,Wuhan Institute of Design and Sciences,WuHan 430205,China;College of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,China)
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2022年第7期67-77,87,共12页
Journal of Northeast Agricultural University
基金
国家自然科学基金面上项目(31871830)
湖北省高等学校优秀中青年科技创新团队计划项目(T2021047)
湖北省教育厅科学研究计划指导性项目(B2017366)。
关键词
山楂叶多酚
提取工艺
体外降糖
体内降糖
hawthorn leaf polyphenols
extraction process
in vitro hypoglycemic
in vivo hypoglycemic
