摘要
紫花野菊(Chrysanthemum zawadskii Herbich),又称山菊、西伯利亚菊,是浙江省淳安县高山特色茶用菊花,饮用历史悠久。该菊味微苦,性温和,有清热解毒和消炎降火的功效。2020年5月,淳安县多个苗圃发现一种紫花野菊茎叶部病害,发于幼苗茎基部,或植株的中上部茎,初期表现为病部产生湿润状暗绿色斑点,遇雨水病斑呈水渍状迅速扩展,茎表皮组织发黑腐烂,无臭味,病原细菌沿导管向叶柄和叶脉发展,叶柄及叶脉发黑,叶片瘫软。
Chrysanthemum zawadskii Herbich is widely planted in Chunan County in Zhejiang as an important local cash crop. Soft rot disease of C. zawadskii Herbich had been found from 2017 to 2019. Symptoms include watermarks on the stems or leaves, softening and sagging and hollowing of the stems, and eventually fallen of the entire plant. Identification of two isolates from the soft rot tissue, ZJ-4-1 and ZJ-4-2, were carried out by observation of the colony morphology, pathogenicity determination, Biolog test, 16 S rDNA and specific sequencing. The results showed that the pathogens were Gram-negative, rod-shaped Pectobacterium with flagellum. Subspecies-specific primers EXPCCF/EXPCCR, Eca1 f/Eca2 r and Br1 f/L1 r identified the isolated strains as Pectobacterium carotovorum subsp. carotovorum(Pcc)based on the active amplification result of primers EXPCCF/EXPCCR and negative results of the two later primers. Systematic evolution analysis based on 16 S rDNA amplification sequence has more than 95% similarity with the reported Pectobacterium carotovorum subsp.carotovorum strains. The consistent result has been obtained based on phylogenetic tree of housekeeping genes(mdh, proA) and Biolog test.
作者
郭方其
叶琪明
吴超
方丽
武军
谢昀烨
王汉荣
GUO Fangqi;YE Qiming;WU Chao;FANG Li;WU Jun;XIE Yunye;WANG Hanrong(The Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;The Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2022年第4期708-711,共4页
Acta Phytopathologica Sinica
基金
浙江省重点研发项目(2019C02025)。
