摘要
为了将粘质沙雷氏菌(Serratia marcescens)应用在松墨天牛(Monochamus alternatus)防治上,对粘质沙雷氏菌的培养基配方进行了优化。通过单因素试验筛选粘质沙雷氏菌发酵培养基的碳源、氮源和无机盐,利用Plackett-Burman试验设计确定3个主要影响因素,通过最陡爬坡试验,结合Box-Behnken Design试验设计及响应面分析法优化培养基组成成分,获得培养基最佳配方与发酵条件:葡萄糖23.27 g·L^(-1)、蚕蛹粉22.37 g·L^(-1)、酵母粉10 g·L^(-1)、硫酸镁2 g·L^(-1)、磷酸氢二钠3 g·L^(-1),氯化钠2 g·L^(-1);在28℃、150 r·min^(-1)条件下发酵36 h,装液量体积分数为50 mL/250 mL,接种量4.73%,pH 7.1~7.3。最后,根据最优培养条件验证发现优化后培养基中生长的菌落数为6.0×10^(9)cfu·mL^(-1),是初始培养基的9.42倍,与模型预测值相符。研究结果为粘质沙雷氏菌的发酵生产提供了理论基础,为松材线虫病(pine wilt disease)的防治提供了新的途径。
In order to develop the application of Serratia marcescens in the control of Monochamus alternatus,the medium formulation of Serratia marcescens was optimized in this study. The carbon source,nitrogen source and inorganic salt of S. marcescens fermentation medium were screened by single factor test,and 3 main influencing factors were determine by Plackett-burman design. The composition of the medium was optimized by the steepest climbing test combined with Box-Behnken Design and response surface analysis,and the optimal medium formula and fermentation conditions were obtained:glucose 23.27 g·L^(-1),soybean cake meal 22.37 g·L^(-1),yeast extract10 g·L^(-1),MgSO_(2) g·L^(-1),NaHPO3 g·L^(-1),NaCl 2 g·L^(-1). At 28 ℃,150 r·min^(-1)for 36 h,the volume fraction of liquid was 50 mL/250 mL,the inoculation amount was 4.73 %,pH 7.1~7.3. Finally,according to the optimal culture conditions,the number of bacteria growing in the optimized medium was 6.0×10^(9)cfu·mL^(-1),9.42 times of the initial medium,which was consistent with the predicted value of the model. These results provided theoretical basis for the fermentation production of S. marcescens and provide a new approach for the control of pine wilt disease.
作者
李金荣
周彤
林一琪
黄祚骅
盛亮菁
张飞萍
吴松青
LI Jinrong;ZHOU Tong;LIN Yiqi;HUANG Zuohua;SHENG Liangjing;ZHANG Feiping;WU Songqing(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Life Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Key Laboratory of Integrated Pest Management in Ecological Forests of Fujian Provincial University,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2022年第8期133-143,共11页
Journal of Agricultural Science and Technology
基金
国家林业和草原局重大应急科技项目(2D202001)
国家自然科学基金项目(U1905201,32171805)
福建省林业科技重点项目(2021FKJ03)
福建省自然科学基金项目(2019J05042)。
关键词
粘质沙雷氏菌
单因素试验
菌落数
响应面分析
松材线虫病
Serratia marcescens
single factor test
colony number
response surface analysis
pine wilt disease
