摘要
结合薄膜分散法、超声水化法和微孔滤膜过滤制备灵芝(Ganoderma lucidum)多糖脂质体。采用单因素实验考察膜材比、药脂比、超声时间和水化时间对灵芝多糖脂质体包封率的影响,通过响应面实验优化制备工艺,得到的最优工艺为膜材比1∶15、药脂比1∶20、超声时间30 min、水化时间20 min。优化工艺制备的灵芝多糖脂质体为淡黄色透明澄清液体,平均包封率为75.73%,平均多糖含量为4.14%。
Liposomes of Ganoderma lucidum polysaccharide were prepared by thin film dispersion, ultrasonic hydration and microporous membrane filtration. The effects of film to material ratio, drug to lipid ratio, ultrasonic time and hydration time on the encapsulation efficiency of G. lucidum polysaccharide liposomes were investigated by single factor experiments. Then the liposome preparation process was optimized by response surface methodology, and the optimal conditions for liposome preparation were as follows : film to material ratio of 1∶15, drug to lipid ratio of 1∶20, ultrasonic time of 30 min and hydration time of 20min. The resultant liposomes were light yellow transparent liquid with an average encapsulation efficiency of 75. 73 % and an average polysaccharide content of 4. 14 %.
作者
龚频
方文静
赵文婧
韩业雯
张知渊
王胜男
陈福欣
GONG Pin;FANG Wenjing;ZHAO Wenjing;HAN Yewen;ZHANG Zhiyuan;WANG Shengnan;CHEN Fuxin(School of Food and Biological Engineering,Shaanxi University of Science&Technology,Xi’an 710021,Shanxi,China;Chemistry and Chemical Engineering,Xi’an University of Science&Technology,Xi’an 710054,Shanxi,China)
出处
《食用菌学报》
CSCD
北大核心
2022年第5期101-108,共8页
Acta Edulis Fungi
基金
西安市未央区科研创新基金(202036)
榆林市科技计划项目(201907)
陕西省科技厅重点产业创新链(群)—农业领域(2021 ZDLNY 04-01)和陕西省科技厅一般项目(2019 GY-138、2020 GY-236、2021 NY-161)。
关键词
灵芝多糖
脂质体
响应面实验
包封率
Ganoderma lucidum polysaccharide
liposome
response surface experiment
encapsulation efficiency
