摘要
目的 探讨同一供体来源的人牙龈干细胞(human gingival mesenchymal stem cells, hGMSCs),人牙髓干细胞(human dental pulp stem cells, hDPSCs)及人牙周膜干细胞(human periodontal ligament stem cells, hPDLSCs)的增殖、成骨成脂分化能力及特定浓度的肿瘤坏死因子-α(tumour necrosis factor-α, TNF-α)对三者性能的影响,进而为牙周组织再生过程中种子细胞的选取及优化提供策略。方法 分离培养并鉴定hGMSCs、hDPSCs和hPDLSCs。将第3代细胞分别于正常和含10 ng/mL TNF-α的成骨、成脂诱导液中连续培养21天,7天时检测ALP(alkaline phosphatase,ALP)水平,21天时茜素红染色测定成骨能力,油红O染色测定成脂能力。结果 三种干细胞中h GMSCs的增殖及成脂能力最强(P<0.05),h PDLSCs的成骨能力最强(P<0.05),hDPSCs在增殖及分化方面表现居中。10 ng/mL TNF-α刺激下,hGMSCs、hDPSCs、h PDLSCs的增殖及成骨成脂分化能力均受到抑制,hPDLSCs受抑制作用最明显(P<0.05),hGMSCs受抑制影响最小(P<0.05)。结论 hGMSCs、hDPSCs、h PDLSCs在体外均具有增殖分化能力,而10 ng/mL的TNF-α对三者的性能有不同程度抑制作用。三者比较而言,hPDLSCs更适用于骨再生研究,但需提高其抵御炎症的能力;对于hGMSCs,在应用过程中要设法提高其分化能力。
Objective To investigate the proliferation, osteogenic and adipogenic differentiation of gingival mesenchymal stem cells(hGMSCs), human dental pulp stem cells(hDPSCs) and human periodontal ligament stem cells(hPDLSCs) from the same donor and the effect of specific concentrations of tumour necrosis factor-α(TNF-α) on the performance of these, and then provided a strategy for the selection and optimization of seed cells in the process of periodontal tissue regeneration. Methods Isolation culture and identification of hGMSCs, hDPSCs and hPDLSCs. The third-generation stem cells were cultured in normal and 10ng/mL TNF-α-containing osteogenic and adipogenic induction medium for 21 days respectively. Detect the level of ALP at 7days. At 21 days, alizarin red staining was used to determine the osteogenic ability, and oil red O staining was used to determine the adipogenic ability.Results Among the three stem cells, hGMSCs had the strongest proliferation and adipogenic ability(P <0.05),hPDLSCs had the strongest osteogenic ability(P<0.05), and hDPSCs had the middle performance in proliferation and differentiation. 10 ng/mL TNF-α had different degrees of inhibitory effect on the proliferation and differentiation of three stem cells, among which h PDLSCs had the most significant inhibitory effect(P<0.05), and hGMSCs had the least effect(P <0.05). Conclusion hGMSCs, hDPSCs, and h PDLSCs all have the ability to proliferate and differentiate in vitro, and 10 ng/mL TNF-α have different degrees of inhibiting effects on their properties. hPDLSCs are relatively more suitable for bone regeneration research, but their ability to resist inflammation needs to be improved. As for hGMSCs, it is necessary to improve their differentiation ability in the application.
作者
赵琦
齐霞
李雅
鲁晓琪
李淑娟
ZHAO Qi;QI Xia;LI Ya;LU Xiaoqi;LI Shujuan(Department of Periodontology(I)Hebei Key Laboralory of Stomaology,Hebei Clinical Research Center for Oral Diseases,School and Hospital of Stomatology,Hebei Medical University,Shjiazhuang 050017)
出处
《现代口腔医学杂志》
CAS
2022年第3期163-168,共6页
Journal of Modern Stomatology
基金
河北省卫健委2019年度政府资助省级临床医学优秀人才培养和基础课题研究项目(2019061441-2)
2021年河北省财政厅老年病防治科研项目(361029)。
关键词
牙龈干细胞
牙髓干细胞
牙周膜干细胞
增殖分化
TNF-Α
Gingival mesenchymal stem cells
Dental pulp stem cells
Periodontal ligament stem cells
Proliferation and differentiation
TNF-α
