摘要
为获得大花卷丹百合的优质种苗,促进其规模化生产,以大花卷丹百合的鳞片为外植体,采用MS、N_(6)、B_(5)培养基,添加激素NAA、6-BA、GA_(3),进行了大花卷丹百合的快繁技术研究。结果表明,外植体在N_(6)培养基中产生愈伤组织最快、效果最好。愈伤组织及不定芽诱导最佳培养基配方为N_(6)+1.0 mg/L 6-BA+0.1 mg/L NAA;不定芽增殖最佳培养基为N_(6)+0.5 mg/L 6-BA+0.1 mg/L NAA+1.0 mg/L GA_(3)。NAA诱导小鳞茎效果最佳,最适培养基为N_(6)+1.0 mg/L NAA;促进小鳞茎膨大生根要在诱导鳞茎的基础上加入IBA,即最适培养基为N_(6)+0.1 mg/L NAA+2.0 mg/L IBA。
To obtain the premium seedlings of Lilium leichtlinii var.maximowiczii(Regel)Baker and to promote its large-scale production,using scales as the explant,MS,N_(6)and B_(5) media with the addition of NAA,6-BA and GA_(3)were applied to conduct the study on techniques of rapid propagation for the tissue culture of Lilium leichtlinii var.maximowiczii(Regel)Baker.Results showed that N_(6)medium was the quickest and the best medium to induce the callus formation,optimum medium for the induction of callus and adventitious buds was N_(6)plus 1.0 mg/L 6-BA plus 0.1 mg/L NAA.The optimum medium for adventitious bud proliferation was N_(6)plus 0.5 mg/L 6-BA plus 0.1 mg/L NAA plus 1.0 mg/L GA_(3).NAA showed the best effect in inducing small bulblets and the optimum medium was N_(6)plus 1.0 mg/L NAA whereas IBA ought to be added to promote the rooting of bulblet enlargement with the optimum medium selected as N_(6)plus 1.0 mg/L NAA plus 2.0 mg/L IBA.
作者
朱岩
王红
代宁
郑敏学
杨蕾
马艳丽
ZHU Yan;WANG Hong;DAI Ning;ZHENG Minxue;YANG Lei;MA Yanli(College of Landscape Architecture,Changchun University,Changchun Jilin 130012,China)
出处
《甘肃农业科技》
2022年第9期76-80,共5页
Gansu Agricultural Science and Technology
基金
吉林省科技厅科技计划项目(212566YY010286378)。
关键词
大花卷丹百合
组织培养
快繁技术
Lilium leichtlinii var.maximowiczii(Regel)Baker
Tissue culture
Rapid propagation technique
