摘要
为研究脱落酸(abscisic acid,ABA)及抑制剂钨酸钠(Na_(2)WoO_(4))处理对发芽玉米中类胡萝卜素合成的影响,以‘苏玉29’黄色玉米为材料,将其进行不同质量浓度外源ABA浸泡处理后进行发芽,测定发芽玉米中可溶性蛋白、游离氨基酸、脯氨酸、VC、总酚、抗氧化酶活性、抗氧化能力、类胡萝卜素含量及类胡萝卜素合成基因表达量。结果表明,与对照组相比,ABA处理能提高发芽玉米中可溶性蛋白、游离氨基酸、脯氨酸、VC、总酚的含量和内源ABA含量;5 mg/L ABA条件下,类胡萝卜素含量达到最高,叶黄素和玉米黄质分别较对照组提高了27.6%和20.1%,而Na_(2)WoO_(4)会抑制内源ABA的合成,同时降低类胡萝卜素含量。实时聚合酶链式反应结果表明,ABA处理能够显著提高发芽玉米类胡萝卜素合成基因的表达量。同时,发芽玉米籽粒中抗氧化酶活性及抗氧化能力明显增强。综上,外源ABA处理能够促进发芽玉米内源ABA合成,提高发芽玉米类胡萝卜素合成基因的表达,促进发芽玉米中类胡萝卜素的合成,增强发芽玉米抗氧化能力,提高发芽玉米籽粒的营养品质。
This work was undertaken to study the effects of abscisic acid(ABA)and sodium tungstate(Na_(2)WoO_(4))as an ABA synthesis inhibitor on carotenoid synthesis in germinated maize.Yellow maize kernels(cv.‘Suyu 29’)was soaked in different concentrations of exogenous ABA and germinated.The contents of soluble protein,free amino acids,proline,vitamin C,total phenols and carotenoid,antioxidant enzyme activity,antioxidant capacity,and the expression of the genes associated with carotenoid synthesis in germinated maize were determined.The results showed that ABA increased the contents of soluble protein,free amino acids,proline,vitamin C,total phenols and endogenous ABA in germinated maize compared with the untreated control group.At an ABA concentration of 5 mg/L,the carotenoid content reached the highest level,and the levels of lutein and zeaxanthin increased by 27.6%and 20.1%,respectively,compared with the control group.However,Na_(2)WoO_(4)inhibited the synthesis of endogenous ABA and reduced the content of carotenoids.Quantitative fluorescence polymerase chain reaction(PCR)results showed that ABA treatment could significantly increase the expression of the genes associated with carotenoid synthesis in germinated maize.Meanwhile,the antioxidant enzyme activity and antioxidant capacity were significantly enhanced.In summary,exogenous ABA treatment can promote endogenous ABA synthesis in germinated maize,and increase the expression of the genes associated with carotenoid synthesis and antioxidant capacity,thus improving nutritional quality.
作者
徐昊
何伟伟
李大婧
包怡红
罗浩
王凡予
张钟元
XU Hao;HE Weiwei;LI Dajing;BAO Yihong;LUO Hao;WANG Fanyu;ZHANG Zhongyuan(College of Forestry,Northeast Forestry University,Harbin 150040,China;Institute of Agro-product Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第18期1-8,共8页
Food Science
基金
国家自然科学基金青年科学基金项目(31901710)
国家自然科学基金面上项目(31771984)。
关键词
脱落酸
钨酸钠
发芽玉米
类胡萝卜素
营养品质
abscisic acid
sodium tungstate
germinated maize
carotenoids
nutritional quality
