摘要
为了给燕山板栗资源保护、开发和科学利用提供数据基础和依据,对燕山地区11个群体的149份板栗资源的28个叶片表型性状进行了多样性研究。结果表明:(1)在群体间23个数量表型性状中仅有4个性状存在显著或极显著差异(P<0.05或P<0.01),5个质量性状均无显著差异,但在群体内所有数量性状均存在极显著差异(P<0.01),说明燕山板栗叶片表型在群体间变异处于较低水平,在群体内存在丰富变异。(2)群体间叶片表型分化系数V_(ST)为7.80%,远小于群体内变异在总变异中的占比(92.20%),认为燕山板栗叶片表型变异主要来源于群体内。(3)燕山板栗28个叶片表型性状的平均变异系数(CV)和多样性指数(H’)分别为15.91%和1.24,5个质量性状中以叶片形状的CV和H’最高(34.44%,0.82)、叶缘特征最低(只有具锯齿表型);23个数量性状的CV在4.52%(气孔宽)~28.32%(锯齿深度)之间,H’在1.97(叶片长/宽)~2.09(叶柄长)之间。生理指标、外观形态和解剖结构这3类性状的平均H’分别为2.05、2.04和2.04,CV为外观形态(12.61%)>生理性状(12.53%)>解剖结构(10.29%)。(4)叶厚、栅栏组织厚度、叶片面积、叶片长/宽、气孔长、气孔密度、比叶质量、锯齿与叶缘间角度和锯齿深度这9个表型性状含有丰富的遗传信息,可作为燕山板栗叶片多样性分析的典型性状。(5)基于9个典型叶片性状,在欧氏距离为5时可将149份燕山板栗资源划分为5个类群。
In order to provide data basis and theory reference for the protection,development and utilization of Yanshan chestnut resources.The diversity of 28 leaf phenotypic traits(five quality traits and 23 quantitative traits)of 149 germplasm resources from 11 Yanshan populations was studied.The results showed that:(1)There were significant differences(P<0.01)in 28 leaves quantitative traits within the population,and only four quantitative traits had significant or extremely significant differences among populations.There were no significant differences in five quality traits among populations,indicated that the phenotypic variation of Yanshan chestnut leaves was at a low level among populations,and there were abundant variations within populations.(2)The average of phenotypic differentiation coefficient among populations were 7.80%,which was much less than that within populations(92.20%).It showed that the variation within populations was the main source of variation.(3)The average variation coefficient(CV)and the Shannon-Wiener index(H’)of leave phenotypic traits were 15.91%and 1.24,respectively.Among the five quality traits,the variation coefficient(CV)and Shannon-Wiener index(H’)of leaf shape were the highest(34.44%,0.82),the variation coefficient(CV)and Shannon-Wiener index(H’)of leaf margin character were the lowest(0,0).The variation coefficient(CV)of 23 quantitative traits ranged from 4.52%(Stoma width)to 28.32%(sawtooth depth),and the Shannon-Wiener index(H’)ranged from 1.97(leaf length/width)to 2.09(Petiole length).The average Shannon-Wiener index(H’)of physiological,appearance and anatomical structure were 2.05,2.04 and 2.04,respectively,and the average variation coefficient(CV)from high to low was 12.61%(appearance)>12.53%(physiological)>10.29%(anatomical structure).(4)The nine quantitative traits(leaf thickness,thickness of palisade tissue,leaf area,leaf length/width,stoma length,stoma density,leaf mass per area,the angle between sawtooth and leaf margin and sawtooth depth)contained abundant genetic information,which could be used as typical traits for phenotypic diversity analysis of Yanshan chestnut leaves.(5)Based on nine typical leaf traits,149 chestnut resources were clustered into five groups at the Euclidean distance of five.
作者
郭燕
张树航
李颖
张馨方
王英杰
王广鹏
GUO Yan;ZHANG Shuhang;LI Ying;ZHANG Xinfang;WANG Yingjie;WANG Guangpeng(Changli Institute of Pomology,Hebei Academy of Agricultural and Forestry Sciences,Changli,Hebei 066600,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2022年第8期1673-1688,共16页
Acta Horticulturae Sinica
基金
河北省科技计划项目(21326304D-3)
河北省自然科学基金项目(C2020301053)。
关键词
板栗
资源
燕山地区
叶片
表型性状
多样性
chestnut
resource
Yanshan
leaf
phenotypic trait
phenotypic diversity
