摘要
目的 探讨二甲双胍对具核梭杆菌(Fn)与结直肠癌(CRC)细胞增殖和E-cadherin蛋白表达的影响。方法 采用MTT法测定CRC细胞24 h、48 h、72 h的增殖能力。用Western blotting检测法检测各组E-cadherin蛋白表达情况。结果 二甲双胍与联合组SW480细胞增殖活力明显下调(24 h:P=0.002;48 h:P=0.000;72 h:P=0.000),二甲双胍组和联合组E-cadherin蛋白表达量较对照组和Fn组均明显升高(P<0.05)。结论 二甲双胍可能通过促使肿瘤细胞与E-cadherin结合,抑制Fn附着和侵入上皮细胞,从而降低CRC的发生和浸润转移。
Objective To investigate the effects of metformin on Clostridium nucleatum(FN) and the proliferation and E-cadherin protein expression of colorectal cancer(CRC) cells. Methods MTT assay was used to determine the proliferation of colorectal cancer cells. Western blotting was used to detect the expression of E-Cadherin protein in each group for statistical analysis. Results The proliferation activity of SW480 cells in metformin and combination group were significantly down-regulated(24 h: P=0.002;48 h: P=0.000;72 h: P=0.000);the expression of e-cadherin protein in metformin group and combined group were significantly lower than that in control group and Fn group(P<0.05). Conclusion Metformin may inhibit FN attachment and invasion into epithelial cells by promoting the combination of tumor cells with E-cadherin, thereby reducing the occurrence, infiltration and metastasis of CRC.
作者
魏攀凤
张小燕
WEI Pan-Feng;ZHANG Xiao-Yan(Department of Basic Medical Sciences,Quanzhou Medical College,Quanzhou 362000,China)
出处
《中国药物经济学》
2022年第8期104-106,110,共4页
China Journal of Pharmaceutical Economics
基金
福建省泉州市科技计划项目(2018Z174)。
关键词
二甲双胍
具核梭杆菌
结直肠癌
Metformin
Fusobacterium nucleatum
Colorectal cancer
