摘要
【目的】构建棕榈藤材纤维细胞壁结构模型,扩大棕榈藤材的用途,提高棕榈藤材的利用率。【方法】选择高地钩叶藤为研究对象,对其高2 m处截取试样,经软化、聚乙二醇(PEG)包埋、切片、室温下经硼氢化钠(NaBH_(4))溶液浸泡后,取出切片,用蒸馏水洗净,固定在载玻片上并置于拉曼显微镜下,利用显微共聚焦拉曼光谱仪依次对纤维细胞角隅(CC)、复合胞间层(CML)、次生壁外层(S_(1))、次生壁中层(S_(2))及次生壁内层(S_(3))采用逐点扫描显微探针成像方法获取光谱数据集,再利用LabSpec5软件进行处理,从而测得纤维细胞不同形态区域细胞壁的化学成分浓度。【结果】纤维S_(2)的纤维素浓度最高,CC最低;相反,CC木质素浓度最高,S_(2)木质素浓度最低;CML纤维素和木质素浓度介于S_(2)和CC之间。纤维细胞S_(1)半纤维素浓度最高,S_(2)和S_(3)均较低,CML和CC最低。S_(3)、S_(1)纤维素的浓度差异较小,而木质素与半纤维素浓度的差异较大;其中,S_(1)木质素和半纤维素的浓度高于S_(2)和S_(3)。【结论】高地钩叶藤纤维细胞壁次生壁(S)纤维素、半纤维素浓度高于CML,而木质素浓度低于CML;S_(1)、S_(2)和S_(3)纤维素浓度相差不大,但S_(1)木质素和半纤维素的浓度高于S_(2)和S_(3)。
【Objective】This article aimed to construct the cell wall structure model of rattan fiber,to expand the use and improve the utilization rate of rattan.【Method】Plectocomia himalayana was selected as the research material,and the samples were cut at a height of 2 m,softened,embedded with PEG,sliced and soaked in NaBH_(4) solution at room temperature.Then the slice was washed with distilled water,fixed on the glass slide and placed under the Raman microscope.The Microscopic confocal Raman spectrometer was used to obtain the spectral data sets of the fiber cell corner(CC),the compound middle layer(CML),the outer layer of secondary wall(S_(1)),the middle layer of the secondary wall(S_(2))and the inner layer of the secondary wall(S_(3))via point-by-point scanning microprobe imaging method.The concentration of chemical components of the cell wall in different morphological regions of fibers was obtained after the data processing by LabSpec5 software.【Result】The highest concentration of cellulose was in S_(2) of fiber,and the lowest was in CC.On the contrary,the lignin concentration was highest in CC,and the lowest concentration was in S_(2).Both the cellulose concentration and lignin concentration of CML were between S_(2) and CC.The highest concentration of hemicellulose was in S_(1),and the hemicellulose concentration was low in both S_(2) and S_(3),which was the lowest in CML and CC.The difference in cellulose concentration between S_(1) and S_(3) was small,while the differences in lignin and hemicellulose concentration between the two were relatively large.Among them,the concentrations of lignin and hemicellulose of S_(1) were larger than those of S_(2) and S_(3).【Conclusion】The concentration of cellulose and hemicellulose of the secondary wall(S)of fiber cells is higher than that of CML,which lignin concentration is lower than that of CML.The cellulose concentrations of S_(1),S_(2) and S_(3) are similar,but the concentrations of lignin and hemicellulose of S_(1) are higher than those of S_(2) and S_(3).
作者
汪佑宏
崔浩然
张菲菲
江泽慧
余林鹏
杨明亮
马建锋
田根林
WANG Youhong;CUI Haoran;ZHANG Feifei;JIANG Zehui;YU Linpeng;YANG Mingliang;MA Jianfeng;TIAN Genlin(School of Forestry and Landscape Architecture,Anhui Agricultural University,Hefei 230036,Anhui,China;Huangshan Huasu New Material Science&Technology Co.Ltd.,Huangshan 245900,Anhui,China;International Centre for Bamboo and Rattan,Beijing 100102,China)
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2022年第9期1-7,共7页
Journal of Central South University of Forestry & Technology
基金
国家自然科学基金项目(31570553,31800476)。
关键词
高地钩叶藤
纤维
细胞壁
拉曼光谱
化学成分
微区分布
Plectocomia himalayana
fiber
cell wall
raman spectra
chemical composition
microdistribution
